{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:37:18Z","timestamp":1753879038083,"version":"3.41.2"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T00:00:00Z","timestamp":1720051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Coccidiosis is one of the most prevalent diseases found in local rabbits (<jats:italic>Oryctolagus cuniculus<\/jats:italic>), which is caused by the <jats:italic>Eimeria<\/jats:italic>. The study aimed to more reliably identify <jats:italic>Eimeria<\/jats:italic> species (<jats:italic>Eimeria magna<\/jats:italic>) infecting Local Rabbits in Alkarg City, Saudi Arabia, based the method on the molecular properties and morphological and molecular biological techniques. Sub\u2010spheroidal oocysts measuring 21\u201327\u2009\u00d7\u200912\u201316 (24\u2009\u00d7\u200914.4) \u03bcm (20\u2009n) and with a length\/width (L\/W) ratio of 0.9\u20131.1 (1.0) were identified by microscopic analysis of a fecal sample. Oocysts feature a bi\u2010layered wall that is 1.0\u20131.2 (1.1) \u03bcm thick. About two\u2010thirds of the wall's thickness is made up of a smooth outer layer. A polar granule is present, but neither a micropyle nor an oocyst residuum is present. The ovoidal sporozoites measure 15\u201318\u2009\u00d7\u20098\u201311 (16.5\u2009\u00d7\u20099.5) \u03bcm, have an L\/W ratio of 1.6\u20131.8 (1.7), and take up around 21% of the oocyst's total surface. The mean size of the sub\u2010Stieda body is 1.4\u2009\u00d7\u20092.3\u2009\u03bcm, while the average size of the Stieda body is 0.9\u2009\u00d7\u20091.8\u2009\u03bcm. The para\u2010Stieda body is lacking. Sporocyst residuum appears membrane\u2010bound and has an uneven form made up of several granules. With two refractile bodies below the striations and pronounced striations at the more pointed end, sporozoites are vermiform, measuring an average of 11.6\u2009\u00d7\u20094.0\u2009\u03bcm. The results of the sequencing for the 18S rDNA gene confirmed the species of Eimeria parasites found in the host (rabbits). The current parasite species is closely related to the previously described and deposited <jats:italic>E.\u2009magna<\/jats:italic> and deeply embedded in the genus <jats:italic>Eimeria<\/jats:italic> (family Eimeriidae). According to the findings, single oocyst molecular identification of <jats:italic>Eimeria<\/jats:italic> may be accomplished through consistent use of the morphological and molecular results. It is possible to draw the conclusion that the current research supplies relevant facts that help assess the potential infection and future control measures against rabbit coccidiosis to reduce the financial losses that can be incurred by the rabbit industry in Saudi Arabia.<\/jats:p>","DOI":"10.1111\/jeu.13044","type":"journal-article","created":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T08:55:59Z","timestamp":1720083359000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Morphological and molecular characterization of <i>Eimeria magna<\/i> infecting local rabbit (<i>Oryctolagus cuniculus<\/i>) in Alkarg City, Saudi Arabia"],"prefix":"10.1111","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3717-6424","authenticated-orcid":false,"given":"Mutee","family":"Murshed","sequence":"first","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9727-3221","authenticated-orcid":false,"given":"Mohammed","family":"Mares","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3675-1397","authenticated-orcid":false,"given":"Hossam M. A.","family":"Aljawdah","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5457-2555","authenticated-orcid":false,"given":"Osama B.","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4204-3124","authenticated-orcid":false,"given":"Saleh","family":"Al\u2010Quraishy","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,4]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"1329","article-title":"Prevalence of coccidia (Eimeria spp.) infection in domestic rabbits, Oryctolagus cuniculus, in Riyadh, Saudi Arabia","volume":"45","author":"Abdel\u2010Baki A.\u2010A.S.","year":"2013","journal-title":"Pakistan Journal of Zoology"},{"key":"e_1_2_8_3_1","first-page":"30","article-title":"Hepatic coccidiosis of the domestic rabbit (Oryctolagus cuniculus domesticus L.) in Saudi Arabia","volume":"3","author":"Al\u2010Mathal E.M.","year":"2008","journal-title":"World Journal of Zoology"},{"key":"e_1_2_8_4_1","first-page":"1","article-title":"Coccidiosis in rabbits from Saudi Arabia 1\u2010exogenous stages of different Eimeria spp","volume":"42","author":"Bashtar A.\u2010R.","year":"2003","journal-title":"Journal\u2010Egyptian German Society of Zoology"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.11.015"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1996.0017"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.5.1.143"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0010414002035004002"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.54203\/scil.2020.wvj60"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-011-2479-0"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.17576\/jsm-2018-4701-02"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201300286"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352008000500034"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2019.1256-1260"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(87)90012-9"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-022-07580-x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsusc.2016.05.125"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182007004106"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2006.06.036"},{"issue":"1","key":"e_1_2_8_20_1","first-page":"289","article-title":"Morphology and molecular identification of the Eimeria spp. in domestic rabbits","volume":"48","author":"Li H.","year":"2016","journal-title":"Pakistan Journal of Zoology"},{"key":"e_1_2_8_21_1","first-page":"6","article-title":"A guide to laboratory techniques used in the study and diagnosis of avian coccidiosis","volume":"201","author":"Long P.","year":"1976","journal-title":"Folia Veterinaria Latina"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.00384"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci10060400"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph16070928"},{"issue":"12","key":"e_1_2_8_25_1","first-page":"1662","article-title":"A comparison in terms of prevalence, morphological and histopathological changes between Eimeria spp. that infect local rabbits (Oryctolagus cuniculus) in some regions of Saudi Arabia","volume":"57","author":"Murshed M.","year":"2023","journal-title":"Indian Journal of Animal Research"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.8.4806-4813.2003"},{"key":"e_1_2_8_27_1","first-page":"293","article-title":"Secondary metabolites of plants and their role: overview","volume":"9","author":"Pagare S.","year":"2015","journal-title":"Current Trends In Biotechnology And Pharmacy"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(96)00975-2"},{"key":"e_1_2_8_29_1","first-page":"1213","article-title":"How do sporozoites of rabbit Eimeria species reach their target cells? 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