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The amastigote, the replicative intracellular form, is essential for infection persistence in vertebrate hosts. These forms exhibit remarkable adaptability, modulating metabolism and growth according to host cell resource availability. Lipid metabolism plays a critical role in amastigote development, with a strong dependence on host\u2010derived lipids, particularly cholesterol. Although\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T. cruzi<\/jats:italic>\n                    <\/jats:styled-content>\n                    can synthesize some sterols and fatty acids, it also scavenges essential lipids from the host. Changes in host cholesterol metabolism, possibly via SREBPs, may increase intracellular cholesterol levels and promote parasite growth. However, the mechanisms of cholesterol acquisition by amastigotes remain unclear. Here, we investigated cholesterol trafficking from host cells to amastigotes using a fluorescent cholesterol analog. Through confocal and volume electron microscopy, we demonstrated cholesterol uptake by amastigotes, characterized uptake kinetics, and confirmed its importance for parasite development. We also revealed close contact between the host endoplasmic reticulum and the amastigote plasma membrane, consistent with membrane contact sites. Furthermore, we showed that amastigotes can internalize ER\u2010 and Golgi\u2010derived host markers, suggesting a potential route for acquisition of host molecules. These findings provide new insights into lipid acquisition strategies by intracellular\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T. cruzi<\/jats:italic>\n                    <\/jats:styled-content>\n                    amastigotes.\n                  <\/jats:p>","DOI":"10.1111\/jeu.70027","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T06:55:36Z","timestamp":1753080936000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Host Organelle Interactions Facilitate Cholesterol Acquisition by\n                    <i>Trypanosoma cruzi<\/i>\n                    Amastigotes"],"prefix":"10.1111","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3981-7697","authenticated-orcid":false,"given":"Carolina de Lima","family":"Alcantara","sequence":"first","affiliation":[{"name":"Centro de Pesquisas em Medicina de 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