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Although anticoccidial drugs have been effective in controlling coccidiosis, the emergence of drug resistance in\n                    <jats:italic>Eimeria<\/jats:italic>\n                    poses a persistent challenge to disease management. Our previous study revealed differential expression of superoxide dismutase (\n                    <jats:italic>Et<\/jats:italic>\n                    SOD) between drug\u2010sensitive (DS) and drug\u2010resistant\n                    <jats:italic>Eimeria tenella<\/jats:italic>\n                    strains. In this study, qPCR and western blot analyses demonstrated that\n                    <jats:italic>Et<\/jats:italic>\n                    SOD expression was significantly upregulated in diclazuril\u2010resistant (DZR) and maduramicin\u2010resistant (MRR) strains compared to the DS strain, with a progressive increase corresponding to higher drug concentrations. Enzyme activity assays further confirmed that SOD activity was elevated in both resistant strains. Additionally, qPCR results indicated higher\n                    <jats:italic>Et<\/jats:italic>\n                    SOD mRNA levels in second\u2010generation merozoites and unsporulated oocysts than in sporulated oocysts and sporozoites. Indirect immunofluorescence revealed that fluorescence intensity increased during parasite development within host cells. Notably,\n                    <jats:italic>Et<\/jats:italic>\n                    SOD was predominantly localized on the surface of sporozoites and merozoites, as well as on the parasitophorous vacuole membrane (PVM) post\u2010invasion of DF\u20101 cells, suggesting its potential role in host\u2013parasite interactions and immune evasion. Collectively, these findings imply that\n                    <jats:italic>Et<\/jats:italic>\n                    SOD may contribute to drug resistance and facilitate parasite growth and development within host cells.\n                  <\/jats:p>","DOI":"10.1111\/jeu.70057","type":"journal-article","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T08:45:32Z","timestamp":1765183532000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Characterization of Superoxide Dismutase and Its Putative Relationship With Drug Resistance in\n                    <i>Eimeria Tenella<\/i>"],"prefix":"10.1111","volume":"73","author":[{"given":"Xinrui","family":"Xie","sequence":"first","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences Key Laboratory of Animal Parasitology of Ministry of Agriculture  Shanghai People's Republic of 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Agriculture  Shanghai People's Republic of 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