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To investigate cytoskeleton\u2010associated regulation during this proliferative process, we cloned and characterized the \u03b3\u2010tubulin gene (\n                    <jats:italic>Ci\u2010TUBG<\/jats:italic>\n                    ) and \u03b3\u2010tubulin complex protein 3 gene (\n                    <jats:italic>Ci\u2010TUBGCP3<\/jats:italic>\n                    ) from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. irritans<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Sequence analysis showed that Ci\u2010TUBG contains a canonical dual tubulin domain architecture and is highly conserved relative to homologs from\n                    <jats:italic>Ichthyophthirius multifiliis<\/jats:italic>\n                    and\n                    <jats:italic>Tetrahymena thermophila<\/jats:italic>\n                    . Structural modeling and molecular docking suggested a stable putative interaction interface between Ci\u2010TUBG and Ci\u2010TUBGCP3. Spatiotemporal expression analysis and immunofluorescence revealed stage\u2010dependent upregulation and pronounced perinuclear enrichment of Ci\u2010TUBG during tomont division, with persistent perinuclear localization in theronts. RNA interference showed that\n                    <jats:italic>Ci\u2010TUBG<\/jats:italic>\n                    knockdown markedly reduced tomont hatching rate, decreased theront yield, and caused defects in theront morphogenesis accompanied by abnormal nuclear development. These findings indicate that\n                    <jats:italic>Ci\u2010TUBG<\/jats:italic>\n                    is essential for tomont division and theront morphogenesis in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. irritans<\/jats:italic>\n                    <\/jats:styled-content>\n                    and provide a basis for understanding cytoskeleton\u2010associated developmental regulation in this parasitic ciliate.\n                  <\/jats:p>","DOI":"10.1111\/jeu.70106","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T09:51:42Z","timestamp":1782726702000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Stage\u2010Specific Expression and Perinuclear Enrichment of \u03b3\u2010Tubulin During Tomont Development and Theront Morphogenesis in\n                    <i>Cryptocaryon irritans<\/i>"],"prefix":"10.1111","volume":"73","author":[{"given":"Dongqi","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Applied Marine Biotechnology, Ministry of Education; School of Marine Sciences Ningbo University  Ningbo P. 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