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Using shotgun metagenomic time\u2010series data from influent samples of seven WWTPs across a European latitudinal gradient, we analyzed the taxonomic composition and dynamics of bacteria, protists, fungi, and microscopic metazoa. Influent community composition varied with geographic location and season, with a pronounced north\u2013south divergence driven by dominant taxa and stronger seasonal shifts observed at higher latitudes. Cross\u2010domain associations were pervasive, suggesting that co\u2010varying bacterial and eukaryotic components structure the incoming microbial pool. Our findings provide a pan\u2010European baseline for whole\u2010microbiome wastewater surveillance and highlight that influent communities differ regionally and seasonally. These patterns may be relevant for downstream treatment\u2010stage microbiomes, but direct effects on reactor community assembly and treatment performance require targeted sampling across treatment stages.<\/jats:p>","DOI":"10.1111\/jeu.70112","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T09:08:01Z","timestamp":1788512881000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Pan\u2010European Whole\u2010Microbiome Study of Wastewater Influent: Prokaryotes, Protists, Fungi, and Metazoa"],"prefix":"10.1111","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-7704-6813","authenticated-orcid":false,"given":"Niklas","family":"Nett","sequence":"first","affiliation":[{"name":"Terrestrial Ecology, Institute of Zoology, University of Cologne  K\u00f6ln Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8798-0483","authenticated-orcid":false,"given":"Kenneth","family":"Dumack","sequence":"additional","affiliation":[{"name":"Aquatic Ecosystem Analyses, Institute for Integrated Natural Sciences, University of Koblenz  Koblenz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ismejo\/wraf235"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-51957-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.6.3393-3398.2003"},{"key":"e_1_2_8_5_1","first-page":"1006","article-title":"Nematodes in Wastewater Treatment","volume":"35","author":"Calaway W. 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