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Here we describe\n                    <jats:italic>Goussia sairae<\/jats:italic>\n                    n. sp. from the liver of Pacific saury (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Cololabis saira<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) in the Northwestern Pacific, characterized through integrated morphological, histopathological, and molecular analyses. Long\u2010read amplicon sequencing of single\u2010oocyst isolates enabled the first complete mitochondrial genome assembly for a marine\n                    <jats:italic>Goussia<\/jats:italic>\n                    species, revealing two co\u2010existing mitogenome genotypes (99.3% identity) within individual oocysts\u2014the first robust evidence consistent with intra\u2010oocyst mitochondrial heterogeneity in the Eimeriidae. Phylogenetic reconstructions based on nuclear 18S ribosomal RNA and concatenated mitochondrial protein\u2010coding genes robustly supported the polyphyly of\n                    <jats:italic>Goussia<\/jats:italic>\n                    sensu lato, recovering five major lineages. A time\u2010calibrated Bayesian phylogeny dated the marine\u2010freshwater divergence to the Early Jurassic (median 197 million years ago; 95% HPD: 124\u2013275 Mya), congruent with the ancient divergence of their respective teleost host groups. These findings support re\u2010evaluation of the current generic boundaries of\n                    <jats:italic>Goussia<\/jats:italic>\n                    and suggest that the hyper\u2010divergent Epicellular lineage may warrant recognition as a distinct family basal to the Eimeriorina.\n                  <\/jats:p>","DOI":"10.1111\/jeu.70114","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T11:52:52Z","timestamp":1786535572000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Polyphyly and Deep Evolutionary History of Piscine Coccidia: Mitochondrial Genomics of a New\n                    <i>Goussia<\/i>\n                    Species From Pacific Saury (\n                    <i>Cololabis saira<\/i>\n                    )"],"prefix":"10.1111","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2495-678X","authenticated-orcid":false,"given":"Peihang","family":"Hong","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Parasitology, School of Veterinary Medicine, Faculty of Veterinary Science Nippon Veterinary and Life Science University  Tokyo Japan"},{"name":"Department of Veterinary Medicine, College of Veterinary Medicine National Chung Hsing University  Taichung City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1943-5146","authenticated-orcid":false,"given":"Toshihiro","family":"Tokiwa","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Parasitology, School of Veterinary Medicine, Faculty of Veterinary Science Nippon Veterinary and Life Science University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1829-033X","authenticated-orcid":false,"given":"Sijia","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Medicine National Chung Hsing University  Taichung City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6277-2581","authenticated-orcid":false,"given":"Shyun","family":"Chou","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Medicine National Chung Hsing University  Taichung City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2307\/3282166"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005080"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.751775"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2006.3537"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0378\u20101119(00)00386\u20103"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2020.12.004"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2024.04.084"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1006650"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1984\u201029612017053"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao018195"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12992"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.5962\/p.287521"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2289704"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btad311"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463\u20106409.1993.tb00346.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"crossref","unstructured":"Doflein F.1909.\u201cLehrbuch der Protozoenkunde: Eine Darstellung der Naturgeschichte der Protozoen Mit Besonderer Ber\u00fccksichtigung der Parasitischen und Pathogenen Formen.\u201dWith Wellcome Library. 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