{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:03:22Z","timestamp":1790337802786,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2027,8,24]],"date-time":"2027-08-24T00:00:00Z","timestamp":1819065600000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2401946"],"award-info":[{"award-number":["2401946"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Mayorella<\/jats:italic>\n                    species are globally distributed amoeboid protists, yet their diversity remains poorly resolved because of pronounced morphological plasticity and limited molecular data. Freshwater ecosystems of West Africa remain particularly underexplored, with virtually no integrative taxonomic or molecular studies addressing\n                    <jats:italic>Mayorella<\/jats:italic>\n                    and broader microbial eukaryotic diversity. Here, we describe\n                    <jats:italic>Mayorella budamensis<\/jats:italic>\n                    sp. nov. isolated from Budamu Stream, Ghana, using an integrative approach combining live\u2010cell morphology and SSU rDNA phylogenetic analyses. The new species exhibits extensive morphological variation in cell size, shape, locomotion, and floating forms. Phylogenetic analyses place it within a well\u2010supported lineage clustering with geographically diverse but largely unnamed and uncultured lineages. Comparative sequence analyses indicate that\n                    <jats:italic>M. budamensis<\/jats:italic>\n                    is genetically distinct from currently available congeners. A second\n                    <jats:italic>Mayorella<\/jats:italic>\n                    isolate recovered from the same locality, known only from single\u2010cell\u2010derived molecular data, represents an additional evolutionary lineage lacking a cultured isolate and currently associated primarily with environmental sequences. Expanded phylogenetic reconstruction identified up to seven putative clades, most containing unnamed or environmental taxa, revealing substantial cryptic diversity. These findings expand the known geographic and evolutionary diversity of\n                    <jats:italic>Mayorella<\/jats:italic>\n                    in African freshwater ecosystems and provide a provisional framework for future taxonomic and phylogenomic studies.\n                  <\/jats:p>","DOI":"10.1111\/jeu.70118","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:06:28Z","timestamp":1787562388000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Understanding the Evolution and Clade Diversity of\n                    <i>Mayorella<\/i>\n                    (Amoebozoa): Description of a Novel Species From Freshwater Near Cape Coast, Ghana"],"prefix":"10.1111","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2781-2351","authenticated-orcid":false,"given":"Yonas I.","family":"Tekle","sequence":"first","affiliation":[{"name":"Spelman College  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8449-0703","authenticated-orcid":false,"given":"Saron","family":"Ghebrezadik","sequence":"additional","affiliation":[{"name":"Spelman College  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1785-105X","authenticated-orcid":false,"given":"Christon Jairus M.","family":"Racoma","sequence":"additional","affiliation":[{"name":"Spelman College  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2996-9177","authenticated-orcid":false,"given":"Angelicia A.","family":"Greer","sequence":"additional","affiliation":[{"name":"Spelman College  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwaku Oti","family":"Acheampong","sequence":"additional","affiliation":[{"name":"Department of Conservation Biology and Entomology University of Cape Coast  Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7813-2244","authenticated-orcid":false,"given":"Kwaku Brako","family":"Dakwa","sequence":"additional","affiliation":[{"name":"Department of Conservation Biology and Entomology University of Cape Coast  Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jeu.12691"},{"key":"e_1_2_9_3_1","first-page":"415","article-title":"A Previously Unnamed Amoeba, Mayorella Clavella, nov. sp","volume":"58","author":"Bovee E. C.","year":"1951","journal-title":"Proceedings of the Iowa Academy of Science"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2307\/3223706"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00189855"},{"key":"e_1_2_9_6_1","first-page":"178","article-title":"The Lobose Amebas. I. A Key to the Suborder Conopodina Bovee and Jahn, 1966 and Descriptions of Thirteen New and Little Known Mayorella Species","volume":"112","author":"Bovee E. C.","year":"1970","journal-title":"Archiv f\u00fcr Protistenkunde"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9365(81)80027-9"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2004.2705"},{"key":"e_1_2_9_9_1","first-page":"205","article-title":"Introduction of Mayorella Gemmifera Schaeffer, 1926 Into Phylogenetic Studies of Amoebozoa","volume":"47","author":"Dykov\u00e1 I.","year":"2008","journal-title":"Acta Protozoologica"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msg201"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2015.11.010"},{"key":"e_1_2_9_12_1","first-page":"29","article-title":"High Genetic Diversity of Amoebae Belonging to the Genus Mayorella (Amoebozoa, Discosea, Dermamoebida) in Natural Habitats","volume":"57","author":"Glotova A.","year":"2018","journal-title":"Acta Protozoologica"},{"key":"e_1_2_9_13_1","first-page":"927","article-title":"Mayorella Dactylifera sp. n. (Gymnamoebia, Paramoebidae) and an Overview of Marine Mayorella Species","volume":"67","author":"Goodkov A.","year":"1988","journal-title":"Zoologicheski\u012d Zhurnal"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msx281"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4285"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"e_1_2_9_17_1","first-page":"220","article-title":"A Checklist of Amoebozoa Species From Marine and Brackish\u2010Water Biotopes With Notes on Taxonomy, Species Concept and Distribution Patterns","volume":"15","author":"Kudryavtsev A.","year":"2021","journal-title":"Protistology"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu531"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.2307\/2389711"},{"key":"e_1_2_9_20_1","volume-title":"Illustrated Guide to the Protozoa","author":"Lee J. 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(Gymnamoebia), Distinguished by Surface Structures, With Comparisons of Three Species","volume":"17","author":"Page F.","year":"1981","journal-title":"Protist"},{"key":"e_1_2_9_26_1","first-page":"404","article-title":"A Study of Two Mayorella Species and Proposed Union of the Families Mayorellidae and Paramoebidae (Rhizopodea, Amoebida)","volume":"114","author":"Page F. C.","year":"1972","journal-title":"Archiv f\u00fcr Protistenkunde"},{"key":"e_1_2_9_27_1","volume-title":"An Illustrated Key to Freshwater and Soil Amoebae: With Notes on Cultivation and Ecology","author":"Page F. C.","year":"1976"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9365(83)80037-2"},{"key":"e_1_2_9_29_1","volume-title":"Marine Gymnamoebae","author":"Page F. C.","year":"1983"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9365(87)80053-2"},{"key":"e_1_2_9_31_1","volume-title":"A New Key to Freshwater and Soil Gymnamoebae","author":"Page F. C.","year":"1988"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3103\/S0095452723060063"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12261"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.5962\/bhl.title.1711"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.2307\/3224896"},{"key":"e_1_2_9_36_1","first-page":"3","article-title":"Taxonomy of the Amebas","volume":"24","author":"Schaeffer A. A.","year":"1926","journal-title":"Papers From the Department of Marine Biology of the Carnegie Institution of Washington"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.protis.2005.06.002"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.protis.2011.04.004"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.protis.2007.04.004"},{"key":"e_1_2_9_40_1","first-page":"148","article-title":"Guide to the Methods of Study and Identification of Soil Gymnamoebae","volume":"3","author":"Susan B.","year":"2004","journal-title":"Protistology"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/jeu.70032"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0235725"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-026-45864-9"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/jeu.70008"}],"container-title":["Journal of Eukaryotic Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jeu.70118","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jeu.70118","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1111\/jeu.70118","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jeu.70118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T11:38:39Z","timestamp":1790336319000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jeu.70118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,24]]},"references-count":43,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/jeu.70118"],"URL":"https:\/\/doi.org\/10.1111\/jeu.70118","archive":["Portico"],"relation":{},"ISSN":["1066-5234","1550-7408"],"issn-type":[{"value":"1066-5234","type":"print"},{"value":"1550-7408","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,24]]},"assertion":[{"value":"2026-01-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70118"}}