{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:04:19Z","timestamp":1790337859488,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/T008369\/1\/2748026"],"award-info":[{"award-number":["BB\/T008369\/1\/2748026"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Until now, only five photoactive pigments have been identified in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Euglena gracilis<\/jats:italic>\n                    <\/jats:styled-content>\n                    and there has been contradictory reporting of the major carotenoid. The impact of various environmental factors on the carotenoid content of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. gracilis<\/jats:italic>\n                    <\/jats:styled-content>\n                    has not been well studied. Using an improved HPLC UV\u2013VIS method, combined with mass spectrometry, we have now identified 12 photoactive pigments from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. gracilis<\/jats:italic>\n                    <\/jats:styled-content>\n                    extracts and confirmed diadinoxanthin as the major carotenoid produced in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. gracilis<\/jats:italic>\n                    <\/jats:styled-content>\n                    . After testing cultivation media and major carbon sources (addition of glucose\u20142\u201320\u2009g\/L, ethanol\u20140.2%\u20132.0% w\/v), we found that EG: JM with 0.8% ethanol increased diadinoxanthin relative production by 361% (4.29\u2009mg\/L) when compared to standard cultivation (EG media, 24\u2009h light, PPFD 25.9\u2009\u03bcmol\/m\n                    <jats:sup>2<\/jats:sup>\n                    \/s and 25\u00b0C). This investigation also tested a range of light intensities (PPFD 0\u201341.7\u2009\u03bcmol\/m\n                    <jats:sup>2<\/jats:sup>\n                    \/s) and found that increasing light intensity (PPFD to 41.7\u2009\u03bcmol\/m\n                    <jats:sup>2<\/jats:sup>\n                    \/s) increased diadinoxanthin production by a further 36% (5.82\u2009mg\/L), giving an increase of 489% over the standard cultivation conditions.\n                  <\/jats:p>","DOI":"10.1111\/jeu.70124","type":"journal-article","created":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:45:14Z","timestamp":1789188314000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Glow Up: Optimizing Growth Conditions and Carotenoid Profiling in\n                    <scp>\n                      <i>Euglena gracilis<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7838-8218","authenticated-orcid":false,"given":"Oskar","family":"Fields","sequence":"first","affiliation":[{"name":"School of Chemistry University of Nottingham  Nottingham UK"},{"name":"Biodiscovery Institute University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5831-0015","authenticated-orcid":false,"given":"Elsie","family":"Cunliffe","sequence":"additional","affiliation":[{"name":"School of Chemistry University of Nottingham  Nottingham UK"},{"name":"Biodiscovery Institute University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5941-2806","authenticated-orcid":false,"given":"Ellis C.","family":"O'Neill","sequence":"additional","affiliation":[{"name":"School of Chemistry University of Nottingham  Nottingham 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Pharmaceuticals) and Regional 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