{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:03:23Z","timestamp":1790337803358,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["573.695\/2008\u20103"],"award-info":[{"award-number":["573.695\/2008\u20103"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["1274\/2011"],"award-info":[{"award-number":["1274\/2011"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E\u201026\/010.001242\/2016"],"award-info":[{"award-number":["E\u201026\/010.001242\/2016"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E\u201026\/211.613\/2021"],"award-info":[{"award-number":["E\u201026\/211.613\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                    undergoes heat stress during its life cycle. In this study, we performed a transcriptomic analysis of the heat shock response in epimastigotes from different\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T. cruzi<\/jats:italic>\n                    <\/jats:styled-content>\n                    strains. In both clone CL Brener and the Y strain, very few to no statistically significant variations were observed in the transcriptome at 37\u00b0C, whereas differentially expressed genes were observed at 40\u00b0C, with 1018 upregulated and 734 downregulated transcripts in CL Brener and 1426 upregulated and 1223 downregulated transcripts in the Y strain. Upregulated transcripts were enriched in catabolic pathways, whereas downregulated transcripts were enriched in catabolic and anabolic pathways. In addition, the expression pattern of different members of several heat shock protein gene families varied. The expression patterns of members of surface protein gene families, such as trans\u2010sialidases, GP63, mucins, and mucin\u2010associated proteins, also varied, although the physiological significance of these patterns remains unclear. We conclude that, in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T. cruzi<\/jats:italic>\n                    <\/jats:styled-content>\n                    epimastigotes, transcriptome modulation at 37\u00b0C was less pronounced than expected for an 8\u00b0C increase in temperature, whereas at 40\u00b0C, there was functional enrichment in both induced and repressed transcripts, characterizing a classical response to severe heat shock.\n                  <\/jats:p>","DOI":"10.1111\/jeu.70125","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T04:44:51Z","timestamp":1789015491000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Different Transcriptomic Responses After Moderate and Severe Heat Shock in\n                    <i>Trypanosoma cruzi<\/i>\n                    Epimastigotes"],"prefix":"10.1111","volume":"73","author":[{"given":"Thayane","family":"Bottaro","sequence":"first","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana L.","family":"Penha","sequence":"additional","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2998-6999","authenticated-orcid":false,"given":"Rosane","family":"Silva","sequence":"additional","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3370-0586","authenticated-orcid":false,"given":"Tur\u00e1n P.","family":"\u00dcrm\u00e9nyi","sequence":"additional","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1105"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2005.02.004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000177"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576\u2010020\u20100258\u20104"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(87)90006-1"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09221"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071\u2010020\u201004125\u2010y"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041430306"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612023396276"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574\u20106976.2007.00069.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes11101196"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2014.01.006"},{"key":"e_1_2_9_15_1","first-page":"153","article-title":"Heat Shock Protein 70 (HSP70) in Gastric Adaptation to Aspirin in \n                     Helicobacter pylori\n                   Infection","volume":"52","author":"Konturek J. 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P. D. C.Rodrigues R.Silva andE.Rondinelli.2012.\u201cThe Stress Response ofTrypanosoma cruzi.\u201dJ. Requena editor. 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