{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:04:30Z","timestamp":1790337870136,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"am","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["2025\u201004268"],"award-info":[{"award-number":["2025\u201004268"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2119963"],"award-info":[{"award-number":["2119963"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2405455"],"award-info":[{"award-number":["2405455"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Euglenids are microbial flagellates best known for their highly flexible photosynthetic species (e.g.,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Euglena gracilis<\/jats:italic>\n                    <\/jats:styled-content>\n                    ), though many heterotrophic groups remain poorly characterized at both molecular and ultrastructural levels. In particular, rigid heterotrophs such as ploeotids and petalomonads are highly diverse, yet most described species were documented only with light microscopy, largely because they are difficult to cultivate. Here, we establish cultures for three new isolates of ploeotids and petalomonads (\n                    <jats:italic>Hokulea pusilla<\/jats:italic>\n                    comb. nov.,\n                    <jats:italic>Ploeotia protuberantia<\/jats:italic>\n                    sp. nov.,\n                    <jats:italic>Scytomonas flumensis<\/jats:italic>\n                    sp. nov.) and examine them using light microscopy, scanning electron microscopy, and 18S rDNA phylogenetic analyses. These data support the recognition of two new species,\n                    <jats:italic>Ploeotia protuberantia<\/jats:italic>\n                    sp. nov. and\n                    <jats:italic>Scytomonas flumensis<\/jats:italic>\n                    sp. nov., and justify the transfer of\n                    <jats:italic>Ploeotia pusilla<\/jats:italic>\n                    to\n                    <jats:italic>Hokulea pusilla<\/jats:italic>\n                    comb. nov. based on its morphology and its affiliation with\n                    <jats:italic>Hokulea waialensis<\/jats:italic>\n                    in 18S rDNA phylogenies.\n                  <\/jats:p>","DOI":"10.1111\/jeu.70128","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:56:27Z","timestamp":1790146587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phylogenetic Placement and Morphological Description of Three Species of Heterotrophic Euglenids (Euglenozoa) From Arizona:\n                    <scp>\n                      <i>Scytomonas flumensis<\/i>\n                      n. sp.,\n                      <i>Ploeotia protuberantia<\/i>\n                      n. sp., and\n                      <i>Hokulea pusilla<\/i>\n                      comb. nov\n                    <\/scp>"],"prefix":"10.1111","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-8743-4438","authenticated-orcid":false,"given":"Maia V.","family":"Palka","sequence":"first","affiliation":[{"name":"Department of Botany, Beaty Biodiversity Research Centre and Museum University of British Columbia  Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1311-1637","authenticated-orcid":false,"given":"Alfredo","family":"Rodriguez\u2010Ruiz","sequence":"additional","affiliation":[{"name":"Biodesign Center for Mechanisms of Evolution Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3986-3068","authenticated-orcid":false,"given":"Gordon","family":"Lax","sequence":"additional","affiliation":[{"name":"Department of Biology Dalhousie University  Halifax Nova Scotia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yesenia","family":"Garza","sequence":"additional","affiliation":[{"name":"Biodesign Center for Mechanisms of Evolution Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0798-0470","authenticated-orcid":false,"given":"Brian S.","family":"Leander","sequence":"additional","affiliation":[{"name":"Department of Botany, Beaty Biodiversity Research Centre and Museum University of British Columbia  Vancouver British Columbia Canada"},{"name":"Department of Zoology, Beaty Biodiversity Research Centre and Museum University of British Columbia  Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4426-9533","authenticated-orcid":false,"given":"Jeremy G.","family":"Wideman","sequence":"additional","affiliation":[{"name":"Biodesign Center for Mechanisms of Evolution Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Algal Culturing Techniques","author":"Andersen R. 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R. M. V.Palka G.Lax et\u00a0al.2026. \u201cPhylogenomic Placement and Morphological Description of a Novel Phagotrophic Euglenid From Hawaii:Hokulea waialensisn. gen. et sp.\u201d73:e70069.https:\/\/doi.org\/10.1111\/jeu.70069.","DOI":"10.1111\/jeu.70069"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025457801420"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0163962"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(65)90054-6"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.326"},{"issue":"5","key":"e_1_2_9_44_1","article-title":"Investigations of the Feeding Apparatus in a Phagotrophic Euglenoid","volume":"103","author":"Triemer R. 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