{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T04:47:05Z","timestamp":1791780425691,"version":"4.3.4"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["KAKENHI grant no. 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JPMJGX23B0"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["SPRING JPMJSP2139"],"award-info":[{"award-number":["SPRING JPMJSP2139"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Eukaryotic Microbiology"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Under anaerobic conditions,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Euglena gracilis<\/jats:italic>\n                    <\/jats:styled-content>\n                    degrades paramylon and synthesizes wax esters (WEs) while accumulating succinate in the culture supernatant. Succinate can either be converted to succinyl\u2010CoA by succinyl\u2010CoA synthetase (SCS) and enter the propionyl\u2010CoA biosynthetic pathway or accumulate extracellularly. Propionyl\u2010CoA serves as a primer for odd\u2010chain WE synthesis. Prior research revealed that knockout (KO) of the SCS \u03b1\u2010subunit (SCS\u03b1) nearly eliminated odd\u2010chain WE synthesis while preserving overall WE accumulation. In the present study, the effects of targeted modifications at anaerobic metabolic branch points on carbon allocation between WE synthesis and succinate accumulation were examined. SCS\u03b1 KO increased succinate accumulation and markedly reduced odd\u2010chain WEs while largely maintaining total WE levels. Silencing mitochondrial NADP\n                    <jats:sup>+<\/jats:sup>\n                    \u2010dependent malic enzyme (ME), which affects carbon allocation at the malate branch point between WE synthesis and the fumarate\u2010to\u2010succinate pathway, also increased succinate accumulation, although total WE accumulation decreased only slightly. Combining ME silencing with SCS\u03b1 KO increased succinate accumulation to 8.9\u2010fold that of the wild type while retaining more than 75% of the WT total WE level. These findings demonstrate that targeted modification of anaerobic metabolic branch points can redirect carbon allocation toward succinate accumulation without abolishing WE biosynthesis in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. gracilis<\/jats:italic>\n                    <\/jats:styled-content>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/jeu.70134","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T04:26:36Z","timestamp":1791779196000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineering Anaerobic Metabolic Branch Points to Control Carbon Allocation Between Wax Ester Synthesis and Succinate Accumulation in\n                    <scp>\n                      <i>Euglena gracilis<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"73","author":[{"given":"Rikuto","family":"Oishi","sequence":"first","affiliation":[{"name":"Department of Applied Biological Chemistry, Graduate School of Agriculture Osaka Metropolitan University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryunosuke","family":"Katayama","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Chemistry, Graduate School of Agriculture Osaka Metropolitan University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuhiro","family":"Ueda","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Chemistry, Graduate School of Agriculture Osaka Metropolitan University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuji","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Chemistry, Graduate School of Agriculture Osaka Metropolitan University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1639-0560","authenticated-orcid":false,"given":"Masami","family":"Nakazawa","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Chemistry, Graduate School of Agriculture Osaka Metropolitan University  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2010.06.002"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1139\/o59\u2010099"},{"key":"e_1_2_10_4_1","first-page":"581","volume-title":"The Enzymes","author":"Bridger W. 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