{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T10:58:22Z","timestamp":1753354702919,"version":"3.37.3"},"reference-count":53,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,11,4]],"date-time":"2013-11-04T00:00:00Z","timestamp":1383523200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31072244"],"award-info":[{"award-number":["31072244"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"crossref","award":["CARS-46-12"],"award-info":[{"award-number":["CARS-46-12"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Specialized Research Fund for the Doctoral Programme of Higher Education of China","award":["20113104120003"],"award-info":[{"award-number":["20113104120003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:p>In this study, the <jats:italic>dicer<\/jats:italic> gene (designated as <jats:italic>cidicer<\/jats:italic>) was identified and characterized from grass carp <jats:italic>Ctenopharyngodon idella<\/jats:italic>. The complementary <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content>) of <jats:italic><jats:styled-content style=\"fixed-case\">cidicer<\/jats:styled-content><\/jats:italic> contained an open reading frame (<jats:styled-content style=\"fixed-case\">ORF<\/jats:styled-content>) of 5646 nucleotides (nts) encoding a putative protein of 1881 amino acids (aa). The deduced Dicer protein contained all known functional domains identified in other organisms. Tissue tropism analysis indicated that <jats:italic><jats:styled-content style=\"fixed-case\">cidicer<\/jats:styled-content><\/jats:italic> is abundantly expressed in brain, gill, head kidney, liver, spleen, heart, muscle and intestine. In the <jats:italic>C. idella<\/jats:italic> kidney (<jats:styled-content style=\"fixed-case\">CIK<\/jats:styled-content>) cells, messenger <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>) expression of <jats:italic><jats:styled-content style=\"fixed-case\">cidicer<\/jats:styled-content><\/jats:italic> was significantly up\u2010regulated at 24 h (6\u00b736\u2010fold, <jats:italic>P<\/jats:italic> &lt; 0\u00b701) after grass carp reovirus (<jats:styled-content style=\"fixed-case\">GCRV<\/jats:styled-content>) infection, and its transcriptional expression level was also transiently induced to a high level (6\u00b754\u2010fold, <jats:italic>P<\/jats:italic> &lt; 0\u00b701) at 2 h post\u2010stimulation of synthetic double\u2010stranded polyinosinic\u2010polycytidylic potassium salt [poly(I:C)]. <jats:italic>In vivo<\/jats:italic> analysis further showed that the expression of <jats:italic><jats:styled-content style=\"fixed-case\">cidicer<\/jats:styled-content><\/jats:italic> <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> in the liver was induced to a significantly high level at 12 h (8\u00b746\u2010fold, <jats:italic>P<\/jats:italic> &lt; 0\u00b701), and then dropped to normal level at 72 h post\u2010challenge with <jats:styled-content style=\"fixed-case\">GCRV<\/jats:styled-content>. The transcriptional expression pattern of <jats:italic><jats:styled-content style=\"fixed-case\">cidicer<\/jats:styled-content><\/jats:italic> in the spleen tissue was similar to that of liver tissue upon <jats:styled-content style=\"fixed-case\">GCRV<\/jats:styled-content> challenge. These results collectively implied that the identified <jats:italic><jats:styled-content style=\"fixed-case\">cidicer<\/jats:styled-content><\/jats:italic> was an inducible gene responding to viral infection both <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>, and the data would shed light on the interaction between <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> interference (<jats:styled-content style=\"fixed-case\">RNAi<\/jats:styled-content>) antiviral pathway and aquareovirus infection.<\/jats:p>","DOI":"10.1111\/jfb.12219","type":"journal-article","created":{"date-parts":[[2013,11,4]],"date-time":"2013-11-04T12:47:22Z","timestamp":1383569242000},"page":"1234-1248","source":"Crossref","is-referenced-by-count":6,"title":["Molecular cloning and immune responsive expression of a ribonuclease <scp>III<\/scp> orthologue involved in <scp>RNA<\/scp> interference, <i>dicer<\/i>, in grass carp <i>Ctenopharyngodon idella<\/i>"],"prefix":"10.1111","volume":"83","author":[{"given":"X. 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