{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T23:29:54Z","timestamp":1777505394872,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,2,11]],"date-time":"2015-02-11T00:00:00Z","timestamp":1423612800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31302208"],"award-info":[{"award-number":["31302208"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31272681"],"award-info":[{"award-number":["31272681"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"crossref","award":["S2013040016268"],"award-info":[{"award-number":["S2013040016268"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:p>In this study, the complementary <jats:styled-content style=\"fixed-case\">(c)DNA<\/jats:styled-content> sequence encoding orange\u2010spotted grouper <jats:italic>Epinephelus coioides<\/jats:italic> Tak1 (<jats:italic>ectak1<\/jats:italic>) was cloned, which has an open reading frame of 1728 bp that encodes 575 amino acids (aa). Sequence analysis indicated that Ectak1 contains two characteristic conserved domains, <jats:italic>i.e<\/jats:italic>. an N\u2010terminal serine\u2013threonine protein kinase catalytic domain (27\u2013275 aa) and a C\u2010terminal coiled\u2010coil region (499\u2013562 aa). Ectak1 shares high sequence identity with Tak1 from other fish species, especially those of Nile tilapia <jats:italic>Oreochromis niloticus<\/jats:italic> (96%) and zebra mbuna <jats:italic>Maylandia zebra<\/jats:italic> (96%). <jats:italic>ectak1<\/jats:italic> transcripts were expressed broadly in all of the tissues tested, but <jats:italic>ectak1<\/jats:italic> expression was reduced mainly in the local infection sites (skin and gill) after infection with <jats:italic>Cryptocaryon irritans<\/jats:italic>. Intracellular localization analysis showed that Ectak1 was distributed mainly in the cytoplasm. A luciferase reporter assay showed that Ectak1 significantly impaired the <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03ba<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> activity induced by <jats:italic>E. coioides<\/jats:italic> Myd88 and Traf6. Overall, these results suggest that Ectak1 functions to reduce the activity of <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03ba<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> induced by toll\u2010like receptor (<jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>) signal molecules in <jats:styled-content style=\"fixed-case\">HEK\u2010293T<\/jats:styled-content> cells, and it might have an important role in host defences against parasitic infections.<\/jats:p>","DOI":"10.1111\/jfb.12550","type":"journal-article","created":{"date-parts":[[2015,2,13]],"date-time":"2015-02-13T09:46:03Z","timestamp":1423820763000},"page":"417-430","source":"Crossref","is-referenced-by-count":18,"title":["Orange\u2010spotted grouper <i>Epinephelus coioides<\/i> Tak1: molecular identification, expression analysis and functional study"],"prefix":"10.1111","volume":"86","author":[{"given":"Y. W.","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Aquatic Product Safety (Sun Yat\u2010Sen University), Ministry of Education\/State Key Laboratory of Biocontrol The School of Life Sciences, Sun Yat\u2010sen University  135 Xingang West Street, Haizhu District Guangzhou 510275 Guangdong Province The People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Aquatic Product Safety (Sun Yat\u2010Sen University), Ministry of Education\/State Key Laboratory of Biocontrol The School of Life Sciences, Sun Yat\u2010sen University  135 Xingang West Street, Haizhu District Guangzhou 510275 Guangdong Province The People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Aquatic Product Safety (Sun Yat\u2010Sen University), Ministry of Education\/State Key Laboratory of Biocontrol The School of Life Sciences, Sun Yat\u2010sen University  135 Xingang West Street, Haizhu District Guangzhou 510275 Guangdong Province The People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z. Q.","family":"Mo","sequence":"additional","affiliation":[{"name":"College of Animal Science South China Agricultural University  Guangzhou 510642 Guangdong Province PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X. M.","family":"Dan","sequence":"additional","affiliation":[{"name":"College of Animal Science South China Agricultural University  Guangzhou 510642 Guangdong Province PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X. C.","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Bioscience and Biotechnology South China University of Technology  Guangzhou 510006 Guangdong Province PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. X.","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Aquatic Product Safety (Sun Yat\u2010Sen University), Ministry of Education\/State Key Laboratory of Biocontrol The School of Life Sciences, Sun Yat\u2010sen University  135 Xingang West Street, Haizhu District Guangzhou 510275 Guangdong Province The People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,2,11]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.12.010"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/383443a0"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.28.16514"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.076976"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2012.02.017"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1863"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.10.7359"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19980209)75:4<559::AID-IJC11>3.0.CO;2-4"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2009.12.023"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.68.6.909"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2011.01337.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2011.04.012"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2011.11.021"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2013.12.011"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2010.09.006"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/35100529"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02709"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.23.13675"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/18465"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102262200"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)01588-X"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1255"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5265.1179"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1102845"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80244-0"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.7.2475-2484.2001"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(02)01404-3"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2003.10.003"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1355"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80402-1"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5244.2008"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2013.03.003"}],"container-title":["Journal of Fish Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjfb.12550","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjfb.12550","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfb.12550","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T14:07:45Z","timestamp":1696169265000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfb.12550"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["10.1111\/jfb.12550"],"URL":"https:\/\/doi.org\/10.1111\/jfb.12550","archive":["Portico"],"relation":{},"ISSN":["0022-1112","1095-8649"],"issn-type":[{"value":"0022-1112","type":"print"},{"value":"1095-8649","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2]]}}}