{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:46:31Z","timestamp":1790631991394,"version":"4.1.0"},"reference-count":70,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,12,28]],"date-time":"2015-12-28T00:00:00Z","timestamp":1451260800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:p>To understand the effect of low dietary phosphorus (P) intake on the vertebral column of Atlantic salmon <jats:italic>Salmo salar<\/jats:italic>, a primary P deficiency was induced in post\u2010smolts. The dietary P provision was reduced by 50% for a period of 10 weeks under controlled conditions. The animal's skeleton was subsequently analysed by radiology, histological examination, histochemical detection of minerals in bones and scales and chemical mineral analysis. This is the first account of how a primary P deficiency affects the skeleton in <jats:italic>S. salar<\/jats:italic> at the cellular and at the micro\u2010anatomical level. Animals that received the P\u2010deficient diet displayed known signs of P deficiency including reduced growth and soft, pliable opercula. Bone and scale mineral content decreased by <jats:italic>c.<\/jats:italic> 50%. On radiographs, vertebral bodies appear small, undersized and with enlarged intervertebral spaces. Contrary to the X\u2010ray\u2010based diagnosis, the histological examination revealed that vertebral bodies had a regular size and regular internal bone structures; intervertebral spaces were not enlarged. Bone matrix formation was continuous and uninterrupted, albeit without traces of mineralization. Likewise, scale growth continues with regular annuli formation, but new scale matrix remains without minerals. The 10 week long experiment generated a homogeneous osteomalacia of vertebral bodies without apparent induction of skeletal malformations. The experiment shows that bone formation and bone mineralization are, to a large degree, independent processes in the fish examined. Therefore, a deficit in mineralization must not be the only cause of the alterations of the vertebral bone structure observed in farmed <jats:italic>S. salar<\/jats:italic>. It is discussed how the observed uncoupling of bone formation and mineralization helps to better diagnose, understand and prevent P deficiency\u2010related malformations in farmed <jats:italic>S. salar<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jfb.12870","type":"journal-article","created":{"date-parts":[[2015,12,28]],"date-time":"2015-12-28T07:44:56Z","timestamp":1451288696000},"page":"690-708","source":"Crossref","is-referenced-by-count":50,"title":["A primary phosphorus\u2010deficient skeletal phenotype in juvenile Atlantic salmon <i>Salmo salar<\/i>: the uncoupling of bone formation and mineralization"],"prefix":"10.1111","volume":"88","author":[{"given":"P. E.","family":"Witten","sequence":"first","affiliation":[{"name":"Ghent University  Department of Biology, Ledeganckstraat 35 9000 Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A. G.","family":"Owen","sequence":"additional","affiliation":[{"name":"Skretting Aquaculture Research Centre  P. O. Box 48 N\u20104001 Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Fontanillas","sequence":"additional","affiliation":[{"name":"Skretting Aquaculture Research Centre  P. O. Box 48 N\u20104001 Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Soenens","sequence":"additional","affiliation":[{"name":"Ghent University  Department of Biology, Ledeganckstraat 35 9000 Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"McGurk","sequence":"additional","affiliation":[{"name":"Skretting Aquaculture Research Centre  P. O. Box 48 N\u20104001 Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Obach","sequence":"additional","affiliation":[{"name":"Skretting Aquaculture Research Centre  P. O. Box 48 N\u20104001 Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,28]]},"reference":[{"key":"e_1_2_5_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2009.08.033"},{"key":"e_1_2_5_1_3_1","volume-title":"Official methods of analysis of AOAC international","author":"AOAC","year":"1995"},{"key":"e_1_2_5_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1052140209"},{"key":"e_1_2_5_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1062"},{"key":"e_1_2_5_1_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2095.1997.00069.x"},{"key":"e_1_2_5_1_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2095.1998.00095.x"},{"key":"e_1_2_5_1_8_1","unstructured":"Baeverfjord G. Fjelldal P. G. Albrektsen S. Hatlen B. Denstadli V. Ytteborg E. Takle H. Lock E.\u2010J. Berntssen M. H. G. Lundebye A. K. \u00c5sg\u00e5rd T.&Waagb\u00f8 R.(2012). Mineral nutrition and the impact on bone development and skeletal deformities \u2013 a review.Nofima Report37\/2012 1\u201391."},{"key":"e_1_2_5_1_9_1","volume-title":"Chondroid Bone, Secondary Cartilage and Metaplasia","author":"Beresford W. A.","year":"1981"},{"key":"e_1_2_5_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12210-009-0051-y"},{"key":"e_1_2_5_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9290(03)00126-X"},{"key":"e_1_2_5_1_12_1","volume-title":"Vertebrate Paleontology and Evolution","author":"Carroll R. L.","year":"1988"},{"key":"e_1_2_5_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2011.02.032"},{"key":"e_1_2_5_1_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.202.18.2495"},{"key":"e_1_2_5_1_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-003-1470-8"},{"key":"e_1_2_5_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jai.12531"},{"key":"e_1_2_5_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/jai.12518"},{"key":"e_1_2_5_1_18_1","first-page":"119","article-title":"Odontoclasts in the Chinook salmon differ from mammalian odontoclasts by exhibiting a great proportion of cells with high nuclei number","volume":"209","author":"Domon T.","year":"2004","journal-title":"Anatomy and Embryology"},{"key":"e_1_2_5_1_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034072"},{"key":"e_1_2_5_1_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/jai.12509"},{"key":"e_1_2_5_1_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1600-079X.2003.00109.x"},{"key":"e_1_2_5_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2007.03.008"},{"key":"e_1_2_5_1_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2095.2008.00601.x"},{"key":"e_1_2_5_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2011.12.043"},{"key":"e_1_2_5_1_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2095.2011.00918.x"},{"key":"e_1_2_5_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20603"},{"key":"e_1_2_5_1_27_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1730377"},{"key":"e_1_2_5_1_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/jai.12521"},{"key":"e_1_2_5_1_29_1","first-page":"13","volume-title":"Major Transitions in Vertebrate Evolution","author":"Hall B. K.","year":"2007"},{"key":"e_1_2_5_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2006.08.027"},{"key":"e_1_2_5_1_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1052210103"},{"key":"e_1_2_5_1_32_1","volume-title":"The Salmon","author":"Jones J. W.","year":"1959"},{"key":"e_1_2_5_1_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.1998.tb00466.x"},{"key":"e_1_2_5_1_34_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548-8659(1975)104<548:ROASFD>2.0.CO;2"},{"key":"e_1_2_5_1_35_1","first-page":"317","article-title":"\u00dcber die Entstehung und Bildung der Hartsubstanz bei Fischen","volume":"9","author":"Kyle H. M.","year":"1927","journal-title":"Zeitschrift f\u00fcr Mikroskopisch\u2010Anatomische Forschung"},{"key":"e_1_2_5_1_36_1","unstructured":"Lall S. P.&Bishop F. J.(1977). Studies on mineral and protein utilization by Atlantic salmon growth in sea water.Fisheries and Marine Service Technical ReportNo. 688. Halifax NS: Department of Fisheries and Environment."},{"key":"e_1_2_5_1_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/jai.12519"},{"key":"e_1_2_5_1_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12562-013-0626-5"},{"key":"e_1_2_5_1_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/jai.12513"},{"key":"e_1_2_5_1_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0905235106"},{"key":"e_1_2_5_1_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2005.00372.x"},{"key":"e_1_2_5_1_42_1","volume-title":"Nutrient Requirements of Fish","author":"NRC","year":"2011"},{"key":"e_1_2_5_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2012.03.001"},{"key":"e_1_2_5_1_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-012-2175-z"},{"key":"e_1_2_5_1_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.a.10309"},{"key":"e_1_2_5_1_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.1999.tb00645.x"},{"key":"e_1_2_5_1_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/jai.12520"},{"key":"e_1_2_5_1_48_1","volume-title":"Humason's Animal Tissue Techniques","author":"Presnell J. K.","year":"1998"},{"key":"e_1_2_5_1_49_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevEukarGeneExpr.v21.i1.30"},{"key":"e_1_2_5_1_50_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:FISH.0000021778.99011.ce"},{"key":"e_1_2_5_1_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-012-2364-9"},{"key":"e_1_2_5_1_52_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088165"},{"key":"e_1_2_5_1_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(97)00141-5"},{"key":"e_1_2_5_1_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2004.00527.x"},{"key":"e_1_2_5_1_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2007.00890.x"},{"key":"e_1_2_5_1_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2007.00889.x"},{"key":"e_1_2_5_1_57_1","first-page":"3","volume-title":"Bone and Tooth. Proceedings of the First European Bone and Tooth Symposium","author":"Tarlo L. B. H.","year":"1964"},{"key":"e_1_2_5_1_58_1","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/S0021-9258(18)66180-0","article-title":"A microcolorimetric method for the determination of inorganic phosphate","volume":"202","author":"Taussky H. H.","year":"1953","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_5_1_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.10418"},{"key":"e_1_2_5_1_60_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007757321695"},{"key":"e_1_2_5_1_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(97)00300-1"},{"key":"e_1_2_5_1_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-005-2035-9"},{"key":"e_1_2_5_1_63_1","first-page":"19","volume-title":"Vertebrate Paleontology","author":"Witmer L. M.","year":"1995"},{"key":"e_1_2_5_1_64_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.2003.00239.x"},{"key":"e_1_2_5_1_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-185X.2009.00077.x"},{"key":"e_1_2_5_1_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2004.10.018"},{"key":"e_1_2_5_1_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2006.05.005"},{"key":"e_1_2_5_1_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2009.06.037"},{"key":"e_1_2_5_1_69_1","volume-title":"Das Gesetz der Transfor mation der Knochen","author":"Wolff J.","year":"1892"},{"key":"e_1_2_5_1_70_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-010-1069-2"},{"key":"e_1_2_5_2_2_1","unstructured":"Shearer W. M.(1992) Atlantic salmon scale reading guidelines.ICES Cooperative Research Report188. Download available from The Flanders Marine Institute (Vlaams Instituut voor de Zee VILZ) atwww.vliz.be\/imisdocs\/publications\/221120.pdf"}],"container-title":["Journal of Fish Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjfb.12870","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfb.12870","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfb.12870","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfb.12870","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T23:49:40Z","timestamp":1694994580000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfb.12870"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,28]]},"references-count":70,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,2]]}},"alternative-id":["10.1111\/jfb.12870"],"URL":"https:\/\/doi.org\/10.1111\/jfb.12870","archive":["Portico"],"relation":{},"ISSN":["0022-1112","1095-8649"],"issn-type":[{"value":"0022-1112","type":"print"},{"value":"1095-8649","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,28]]}}}