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To accomplish this, three wild strains and one domesticated strain of diploid and triploid <jats:italic>O. mykiss<\/jats:italic> were abruptly transferred from control soft water (City of Vancouver dechlorinated tap water; <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> 6\u00b77; [<jats:styled-content style=\"fixed-case\">CaCO<jats:sub>3<\/jats:sub><\/jats:styled-content>]\u2009&lt;\u200917\u00b79\u2009mg\u2009l<jats:sup>\u22121<\/jats:sup>) to control soft water (handling control), high <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> soft water (<jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> 9\u00b75; [<jats:styled-content style=\"fixed-case\">CaCO<jats:sub>3<\/jats:sub><\/jats:styled-content>]\u2009&lt;\u200917\u00b79\u2009mg\u2009l<jats:sup>\u22121<\/jats:sup>), or high <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> hard water (<jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> 9\u00b75; [<jats:styled-content style=\"fixed-case\">CaCO<jats:sub>3<\/jats:sub><\/jats:styled-content>]\u2009=\u2009320\u2009mg\u2009l<jats:sup>\u22121<\/jats:sup>) followed by sampling at 24\u2009h for physiological measurements. There was a significant effect of ploidy on loss of equilibrium (<jats:styled-content style=\"fixed-case\">LOE<\/jats:styled-content>) over the 24\u2009h exposure, with only triploid <jats:italic>O. mykiss<\/jats:italic> losing equilibrium at high <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> in both soft and hard water. Furthermore, exposure to <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> 9\u00b75 resulted in significant decreases in plasma sodium and chloride, and increases in plasma and brain ammonia with no differences between soft and hard water. There was no significant effect of strain on <jats:styled-content style=\"fixed-case\">LOE<\/jats:styled-content>, but there were significant differences between strains in brain ammonia and plasma cortisol. Overall, there were no clear protective effects of hardness on high <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> exposure in these strains of <jats:italic>O. mykiss<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jfb.13045","type":"journal-article","created":{"date-parts":[[2016,6,22]],"date-time":"2016-06-22T02:33:12Z","timestamp":1466562792000},"page":"1345-1358","source":"Crossref","is-referenced-by-count":4,"title":["Hardness does not affect the physiological responses of wild and domestic strains of diploid and triploid rainbow trout <i>Oncorhynchus mykiss<\/i> to short\u2010term exposure to <scp>pH<\/scp> 9.5"],"prefix":"10.1111","volume":"89","author":[{"given":"W. A.","family":"Thompson","sequence":"first","affiliation":[{"name":"The University of Calgary  507 Campus Drive NW Calgary AB T2N 4V8 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. 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