{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T12:24:32Z","timestamp":1765887872085},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,4,9]],"date-time":"2017-04-09T00:00:00Z","timestamp":1491696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006143","name":"Department of Science and Technology, Government of Kerala","doi-asserted-by":"publisher","award":["SA\/SO\/AS\u201043\/2009"],"award-info":[{"award-number":["SA\/SO\/AS\u201043\/2009"]}],"id":[{"id":"10.13039\/501100006143","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:p>Differential effects of photoperiod and temperature on the temporal modulation of gonadotrophin subunit genes (glycoprotein <jats:italic>\u03b1<\/jats:italic>, <jats:italic>gp\u03b1<\/jats:italic>), follicle\u2010stimulating hormone <jats:italic>\u03b2<\/jats:italic> (<jats:italic>fsh\u03b2<\/jats:italic>) and luteinizing hormone <jats:italic>\u03b2<\/jats:italic> (<jats:italic>lh\u03b2<\/jats:italic>) expression were investigated in the stinging catfish <jats:italic>Heteropneustes fossilis<\/jats:italic>. Female <jats:italic>H. fossilis<\/jats:italic> were exposed to varying photoperiod and temperature conditions for 14 and 28\u2009days in the early preparatory phase of the annual reproductive cycle. Gonadotrophin subunit gene expression, gonado\u2010somatic index (<jats:italic>I<\/jats:italic><jats:sub>G<\/jats:sub>), ovarian histology and plasma steroid hormone levels were evaluated. The exposure of <jats:italic>H. fossilis<\/jats:italic> to long photoperiod (<jats:styled-content style=\"fixed-case\">LP<\/jats:styled-content>) of 16\u2009h light or high temperature (<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content>) at 28\u2009\u00b1\u20092\u00b0 C (mean\u2009\u00b1\u2009<jats:sc>s.e.<\/jats:sc>), alone or in combination, resulted in significant increases in <jats:italic>gp\u03b1<\/jats:italic>, <jats:italic>fsh\u03b2<\/jats:italic> and <jats:italic>lh\u03b2<\/jats:italic> messenger (m)<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> levels, <jats:italic>I<\/jats:italic><jats:sub>G<\/jats:sub>, plasma oestradiol\u201017\u03b2 (<jats:styled-content style=\"fixed-case\">E<jats:sub>2<\/jats:sub><\/jats:styled-content>), testosterone (T) and progesterone (<jats:styled-content style=\"fixed-case\">P<jats:sub>4<\/jats:sub><\/jats:styled-content>) levels. The ovaries were filled with advanced yolky oocytes. On the other hand, the short photoperiod (<jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>) of 8\u2009h light exposure decreased the transcript levels with higher inhibition in the normal temperature (<jats:styled-content style=\"fixed-case\">NT<\/jats:styled-content>) group at 18\u2009\u00b1\u20092\u00b0 C (mean\u2009\u00b1\u2009<jats:sc>s.e.<\/jats:sc>) than the <jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content> group at 28\u2009\u00b1\u20092\u00b0 C. Furthermore, the inhibition reached the highest level in total darkness (<jats:styled-content style=\"fixed-case\">TD<\/jats:styled-content>) of 24\u2009h light deprivation under <jats:styled-content style=\"fixed-case\">NT<\/jats:styled-content> conditions at 18\u2009\u00b1\u20092\u00b0 C. Consequently, the <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TD<\/jats:styled-content> treatments inhibited the <jats:italic>I<\/jats:italic><jats:sub>G<\/jats:sub>, plasma E<jats:sub>2<\/jats:sub> and T levels and ovarian development. The exposure to high temperature at 28\u2009\u00b1\u20092\u00b0 C also modified the short photoperiod effect by elevating plasma E<jats:sub>2<\/jats:sub> level. The plasma T level changed only mildly while the plasma P<jats:sub>4<\/jats:sub> level showed the greatest fluctuations; the level reached the nadir in the SP\u2009+\u2009HT group but increased in the SP\u2009+\u2009NT group on day 28. A two\u2010way ANOVA of the data showed differential effects of photoperiod and temperature; photoperiod produced a highly significant effect on <jats:italic>fsh\u03b2<\/jats:italic> expression while temperature had a highly significant effect both on <jats:italic>lh\u03b2<\/jats:italic> and <jats:italic>gp\u03b1<\/jats:italic> levels. Thus, the differential expression of the <jats:italic>gp\u03b1<\/jats:italic> by the environmental variables ensures temporal synchronization of ovarian development and spawning.<\/jats:p>","DOI":"10.1111\/jfb.13305","type":"journal-article","created":{"date-parts":[[2017,4,10]],"date-time":"2017-04-10T04:37:14Z","timestamp":1491799034000},"page":"2289-2311","source":"Crossref","is-referenced-by-count":21,"title":["Effects of altered photoperiod and temperature on expression levels of gonadotrophin subunit <scp>mRNAs<\/scp> in the female stinging catfish <i><scp>H<\/scp>eteropneustes fossilis<\/i>"],"prefix":"10.1111","volume":"90","author":[{"given":"A.","family":"Acharjee","sequence":"first","affiliation":[{"name":"Department of Zoology Banaras Hindu University  Varanasi 211005 Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Chaube","sequence":"additional","affiliation":[{"name":"Department of Zoology Banaras Hindu University  Varanasi 211005 Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. 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