{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T06:09:56Z","timestamp":1792044596542,"version":"4.4.0"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T00:00:00Z","timestamp":1537747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008363","name":"Sichuan Agricultural University","doi-asserted-by":"publisher","award":["01570702"],"award-info":[{"award-number":["01570702"]}],"id":[{"id":"10.13039\/501100008363","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:p>Apela identified from zebrafish <jats:styled-content style=\"fixed-case\"><jats:italic>Danio rerio<\/jats:italic><\/jats:styled-content> for the first time in 2013 is a novel endogenous peptide ligand for the apelin receptor. To study the role of apela in regulating fish feeding, the complementary (c) DNA sequence of <jats:italic>apela<\/jats:italic> of Siberian sturgeon <jats:styled-content style=\"fixed-case\"><jats:italic>Acipenser baerii<\/jats:italic><\/jats:styled-content> was cloned for the first time. The <jats:italic>apela<\/jats:italic> cDNA fragment of 836\u2009bp was obtained by cloning. The open reading frame (ORF) of <jats:italic>apela<\/jats:italic> was 165\u2009bp encoding a 54 amino acid, including 22 amino acids signal peptide and two proteolytic sites. Phylogenetic tree analysis showed that <jats:styled-content style=\"fixed-case\"><jats:italic>A. baerii<\/jats:italic><\/jats:styled-content> <jats:italic>apela<\/jats:italic> was clustered with mammalian and amphibian sequences. <jats:styled-content style=\"fixed-case\"><jats:italic>A. baerii<\/jats:italic><\/jats:styled-content> <jats:italic>apela<\/jats:italic> messeger (m)RNA was widely distributed in 11 tissues related to feeding, with high expressions in brain, oesophagus and stomach, especially in the brain. The level of <jats:italic>apela<\/jats:italic> mRNA in brain increased significantly after feeding. On the first day of fasting, <jats:italic>apela<\/jats:italic> expression in brain was significantly lower than that of the fed group, but after fasting for 3\u201315\u2009days, the expression of <jats:italic>apela<\/jats:italic> in <jats:styled-content style=\"fixed-case\"><jats:italic>A. baerii<\/jats:italic><\/jats:styled-content> brain was significantly higher than that in the fed group. After refeeding <jats:italic>apela<\/jats:italic> mRNA expression was obviously reduced. These results suggest that <jats:italic>apela<\/jats:italic> plays a bidirectional role in feeding regulation of <jats:styled-content style=\"fixed-case\"><jats:italic>A. baerii<\/jats:italic><\/jats:styled-content>, which may serve as a short\u2010term satiation factor and a long\u2010term hunger factor.<\/jats:p>","DOI":"10.1111\/jfb.13685","type":"journal-article","created":{"date-parts":[[2018,6,29]],"date-time":"2018-06-29T05:25:04Z","timestamp":1530249904000},"page":"609-615","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Characterization, tissue distribution of <i>apela<\/i> and periprandial, fasting and refeeding changes of <i>apela<\/i> mRNA in Siberian sturgeon <i>Acipenser baerii<\/i>"],"prefix":"10.1111","volume":"93","author":[{"given":"Ni","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Aquaculture, College of Animal Science and Technology Sichuan Agricultural University  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