{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T06:08:58Z","timestamp":1768889338629,"version":"3.49.0"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,12,16]],"date-time":"2018-12-16T00:00:00Z","timestamp":1544918400000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:p>The association between major histocompatibility complex (MHC) class IIA polymorphisms and the severity of infection by <jats:styled-content style=\"fixed-case\"><jats:italic>Streptococcus agalactiae<\/jats:italic><\/jats:styled-content> was investigated using 40 susceptible and 40 resistant individuals of Nile tilapia <jats:styled-content style=\"fixed-case\"><jats:italic>Oreochromis niloticus<\/jats:italic><\/jats:styled-content>. Twenty\u2010five alleles were identified from 80 individuals, which belong to 22 major allele types. High polymorphism of <jats:italic>mhcIIa<\/jats:italic> gene and at least two loci were discovered in <jats:styled-content style=\"fixed-case\"><jats:italic>O. niloticus<\/jats:italic><\/jats:styled-content>. In peptide\u2010binding region (PBR) and non\u2010PBR, the ratio of nonsynonymous substitution (<jats:italic>dN<\/jats:italic>) to synonymous substitution (<jats:italic>dS<\/jats:italic>) was 1.294 (&gt;1) and 1.240 (&gt;1), suggesting that the loci are evolving under positive balancing selection. Association analysis showed that the allele, <jats:italic>orni\u2010daa*0501<\/jats:italic>, was significantly associated with resistance to <jats:styled-content style=\"fixed-case\"><jats:italic>S. agalactiae<\/jats:italic><\/jats:styled-content>, while the alleles, <jats:italic>orni\u2010daa*1101<\/jats:italic>, <jats:italic>orni\u2010daa*1301<\/jats:italic>, <jats:italic>orni\u2010daa*1401<\/jats:italic> and <jats:italic>orni\u2010daa*1201<\/jats:italic>, were associated with susceptibility to <jats:styled-content style=\"fixed-case\"><jats:italic>S. agalactiae<\/jats:italic><\/jats:styled-content>. To confirm these correlations, another independent challenge experiment was performed in the Huizhou population of the <jats:styled-content style=\"fixed-case\"><jats:italic>O. niloticus<\/jats:italic><\/jats:styled-content>. The frequency distribution showed that the <jats:italic>orni\u2010daa*1101<\/jats:italic> allele was significantly more frequent in the Huizhou\u2010Susceptible group (HZ\u2010SG) than in the Huizhou\u2010Resistant group (HZ\u2010RG) (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), which was consistent with the first challenge. However, <jats:italic>orni\u2010daa*0501<\/jats:italic> did not present in HZ\u2010SG and HZ\u2010RG and the distribution frequencies of the <jats:italic>orni\u2010daa*1201<\/jats:italic>, <jats:italic>orni\u2010daa*1301<\/jats:italic> and <jats:italic>orni\u2010daa*1401<\/jats:italic> alleles were not significantly more frequent in HZ\u2010SG than in HZ\u2010RG. These results indicate that the <jats:italic>orni\u2010daa*1101<\/jats:italic> allele confers susceptibility to <jats:italic>S. agalactia<\/jats:italic> infection. These results suggest that the diversity of exon 2 of <jats:italic>mcaIIa<\/jats:italic> alleles could be used to explore the association between disease susceptibility or resistance and the multiformity of <jats:italic>mcaIIa<\/jats:italic> and to achieve the molecular\u2010assisted selection of <jats:styled-content style=\"fixed-case\"><jats:italic>O. niloticus<\/jats:italic><\/jats:styled-content> with enhanced disease resistance.<\/jats:p>","DOI":"10.1111\/jfb.13843","type":"journal-article","created":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T03:33:57Z","timestamp":1540179237000},"page":"1207-1215","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["MHC class IIA polymorphisms and their association with resistance\u2013susceptibility to <scp><i>Streptococcus agalactiae<\/i><\/scp> in Nile tilapia, <scp><i>Oreochromis niloticus<\/i><\/scp>"],"prefix":"10.1111","volume":"93","author":[{"given":"Feng\u2010Ying","family":"Gao","sequence":"first","affiliation":[{"name":"Division of Aquaculture and Nutrition, Pearl River Fisheries Research Institute Chinese Academy of Fishery Science  Guangzhou China"},{"name":"Division of Aquaculture and Nutrition, Key Laboratory of Tropical &amp; Subtropical Fishery Resource Application &amp; Cultivation, Ministry of Agriculture  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Aquaculture and Nutrition, Pearl River Fisheries Research Institute Chinese Academy of Fishery Science  Guangzhou China"},{"name":"Division of Aquaculture and Nutrition, Key Laboratory of Tropical &amp; Subtropical Fishery Resource Application &amp; Cultivation, Ministry of Agriculture  Guangzhou China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mai\u2010Xin","family":"Lu","sequence":"additional","affiliation":[{"name":"Division of Aquaculture and Nutrition, Pearl River Fisheries Research Institute Chinese Academy of Fishery Science  Guangzhou China"},{"name":"Division of Aquaculture and Nutrition, Key Laboratory of Tropical &amp; Subtropical Fishery Resource Application &amp; Cultivation, Ministry of Agriculture  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian\u2010Meng","family":"Cao","sequence":"additional","affiliation":[{"name":"Division of Aquaculture and Nutrition, Pearl River Fisheries Research Institute Chinese Academy of Fishery Science  Guangzhou China"},{"name":"Division of Aquaculture and Nutrition, Key Laboratory of Tropical &amp; Subtropical Fishery Resource Application &amp; Cultivation, Ministry of Agriculture  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Gang","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Aquaculture and Nutrition, Pearl River Fisheries Research Institute Chinese Academy of Fishery Science  Guangzhou China"},{"name":"Division of Aquaculture and Nutrition, Key Laboratory of Tropical &amp; Subtropical Fishery Resource Application &amp; Cultivation, Ministry of Agriculture  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Li","family":"Ke","sequence":"additional","affiliation":[{"name":"Division of Aquaculture and Nutrition, Pearl River Fisheries Research Institute Chinese Academy of Fishery Science  Guangzhou China"},{"name":"Division of Aquaculture and Nutrition, Key Laboratory of Tropical &amp; 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