{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T21:51:29Z","timestamp":1784670689392,"version":"3.55.0"},"reference-count":64,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T00:00:00Z","timestamp":1574121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to determine the effect of stress on reproduction and the possible involvement of dopaminergic systems in the reproductive stress response in the mosquitofish <jats:italic>Gambusia affinis<\/jats:italic>. Exposure of fish to aquaculture stressors (four 10\u2009min episodes of stress, each corresponding to a different stressor such as handling, chasing, frequent netting and low water levels), for a period of 30\u2009days caused reduction in the mean numbers of stage I\u2013IV follicles associated with lower number of pregnant females and embryos in most of the developmental stages compared with experimental controls. Besides, increase in the intensity of labelling and the per cent area of tyrosine hydroxylase (TH; a rate\u2010limiting enzyme in the biosynthetic pathway of catecholamines)\u2010 immunoreactive (ir) neurons was observed in the preoptic area (POA) and the nucleus preopticus (NPO) regions of the brain concomitant with reduction in the labelling of gonadotropin releasing hormone\u2013immunoreactive (GnRH\u2010ir) fibres in the proximal pars distalis (PPD) of the pituitary gland in stressed fish compared with experimental controls. Treatment of domperidone (DOM) caused an increase in the number of stage II and V follicles and promoted pregnancy rate concomitant with an increase in the number of embryos at various developmental stages compared with those of experimental controls. Similar treatment to stressed fish caused an increase in the number of stages I\u2013V follicles compared with those in stress alone group. The GnRH fibres showed increased immunolabelling in stress + DOM treated fish compared with stress alone fish. On the other hand, TH\u2010immunoreactivity in the POA and the NPO regions was reduced in stress + DOM treated fish compared with stress\u2010alone group. These results suggest that stress inhibits follicular development and subsequent hatching success through the suppression of GnRH and that the inhibition appears to be mediated through dopamine, for the first time in a viviparous fish.<\/jats:p>","DOI":"10.1111\/jfb.14183","type":"journal-article","created":{"date-parts":[[2019,10,24]],"date-time":"2019-10-24T18:02:35Z","timestamp":1571940155000},"page":"37-48","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Domperidone treatment attenuates stress\u2010induced suppression of reproduction in viviparous mosquitofish <i>Gambusia affinis<\/i>"],"prefix":"10.1111","volume":"96","author":[{"given":"Shilpa K.","family":"Bhat","sequence":"first","affiliation":[{"name":"Department of Studies in Zoology Karnatak University  Dharwad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5662-534X","authenticated-orcid":false,"given":"C. 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