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Motivated by the emerging utility of zebrafish (<jats:italic>Danio rerio<\/jats:italic>) as an animal of choice for the study of musculoskeletal disease, here we report a model of restricted mobility induced osteopenia in adult zebrafish. Aquatic tanks with small cubical compartments to restrict the movement and locomotion of single fish were designed and fabricated for this study. Adult zebrafish were divided into two groups: a normal control (CONT) and a restricted mobility group (RMG) (18 fish\/group). Six fish from each group were euthanized on days 14, 21 and 35 of the movement restriction. By using microcomputed tomography (micro\u2010CT), we assessed bone volume\/tissue volume (BV\/TV) and bone density in the whole skeleton of the fish. Furthermore, we assessed skeletal shape in the vertebrae (radius, length, volume, neural and haemal arch aperture areas, neural and haemal arch angle, and thickness of the intervertebral space), single vertebra bone volume and bone density. Movement restriction significantly decreased vertebral skeletal parameters such as radius, length, volume, arch aperture areas and angles as well as the thickness of the intervertebral space in RMG. Furthermore, restricted mobility significantly (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) decreased BV\/TV and bone density as compared to the CONT group, starting as early as 14\u2009days. By analysing zebrafish from CONT and RMG, we show that micro\u2010CT imaging is a sensitive method to quantify distinct skeletal properties in zebrafish. We further defined the micro\u2010CT parameters which can be used to examine the effects of restricted mobility on the skeleton of the fish. Our findings propose a rapid and effective osteopenia \u201cstabulation\u201d model, which could be used widely for osteoporosis drug screening.<\/jats:p>","DOI":"10.1111\/jfb.14369","type":"journal-article","created":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T07:35:40Z","timestamp":1588923340000},"page":"1031-1038","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Novel model of restricted mobility induced osteopenia in zebrafish"],"prefix":"10.1111","volume":"98","author":[{"given":"Deepak Kumar","family":"Khajuria","sequence":"first","affiliation":[{"name":"The Musculoskeletal Genetics Laboratory, The Azrieli Faculty of Medicine Bar\u2010Ilan University  Safed Israel"},{"name":"Department of Orthopaedics and Rehabilitation Penn State University, College of Medicine  Hershey Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8826-0530","authenticated-orcid":false,"given":"David","family":"Karasik","sequence":"additional","affiliation":[{"name":"The Musculoskeletal Genetics Laboratory, The Azrieli Faculty of Medicine Bar\u2010Ilan University  Safed Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036630"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.204.24.4335"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00006"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10387"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1997.12.10.1547"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1253710"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2017.05.001"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22122068"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(03)00285-X"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2009.07.016"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034072"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.3445"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2006.08.008"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.2337\/db18-0370"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0106030"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.3231"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-012-0305-z"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075787"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.8b02792"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.7b04668"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0482-50042012000300016"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbr.2014.07.007"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2019.02.009"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2451051359"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/bonekey.2013.179"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00070"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crpv.2006.01.002"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpet.19"},{"key":"e_1_2_7_30_1","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1242\/jeb.203.2.193","article-title":"Hydrodynamics of unsteady fish swimming and the effects of body size: comparing the flow fields of fish larvae and adults","volume":"203","author":"Muller U. 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