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The present study aimed at verifying differentially expressed genes (DEGs) in the brain of the threespot wrasse <jats:italic>Halichoeres trimaculatus<\/jats:italic> (tropical species) and the honbera wrasse <jats:italic>Halichoeres tenuispinis<\/jats:italic> (temperate species), both of which were exposed to HP at 30\u2009kPa (possible high\u2010tide stimuli in the field) or 1\u2009kPa (low tide) for 3 or 6 h. A <jats:italic>de novo<\/jats:italic> assembly yielded 174,710 contigs (63,530 contigs were annotated) from the brain of threespot wrasse. Following RNA sequencing, quantitative PCR confirmed DEGs that were upregulated [AT atypical cadherin 2 (<jats:italic>FAT2<\/jats:italic>)] and downregulated [neuronal leucine\u2010rich repeat protein 3 (<jats:italic>LRRN3<\/jats:italic>), dual specificity tyrosine phosphorylation\u2010regulated kinase 1 (<jats:italic>DYRK<\/jats:italic>), mitogen\u2010activated protein kinase kinase 1 (<jats:italic>MAP2K1<\/jats:italic>) and phosphoinositide 3 kinase (<jats:italic>PI3K<\/jats:italic>)]. The effect of HP on the transcription of these DEGs (except for <jats:italic>MAP2K1<\/jats:italic>) disappeared within 6 h, suggesting that HP is a transitory stimulus occurring at the beginning of the tidal cycle. Similar DEG transcription was observed in the brain of honbera wrasse maintained under HP for 6 h. <jats:italic>In situ<\/jats:italic> hybridization of the brain of the threespot wrasse revealed that strong signals of <jats:italic>MPA2K1<\/jats:italic> were seen in the telencephalon, diencephalon and pituitary, whereas those of <jats:italic>PI3K<\/jats:italic> were seen in the telencephalon, diencephalon and medulla oblongata. This result suggests that these kinases are involved in sensory function (telencephalon), somatic and visceral function (medullar oblongata) and the neuroendocrine system (diencephalon and pituitary), all of which were related to changes in HP stimuli. Following HP exposure, the transcription of <jats:italic>c\u2010fos<\/jats:italic> increased in the pituitary of honbera wrasse, suggesting that external stimuli directly or indirectly activate hormone synthesis at the hypothalamic\u2013pituitary\u2013gonadal axis. It is concluded that HP alters gene expression in relation to neural development and function in the central nervous system and plays a role in exerting tidal\u2010related reproduction and feeding in wrasses.<\/jats:p>","DOI":"10.1111\/jfb.14458","type":"journal-article","created":{"date-parts":[[2020,7,10]],"date-time":"2020-07-10T11:40:09Z","timestamp":1594381209000},"page":"1027-1038","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Verification of differentially expressed genes in relation to hydrostatic pressure in the brain of two wrasse species with high\u2010tide preference in spawning"],"prefix":"10.1111","volume":"97","author":[{"given":"Shingo","family":"Udagawa","sequence":"first","affiliation":[{"name":"Department of Marine and Environmental Science Graduate School of Engineering and Science, University of the Ryukyus  Okinawa 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