{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T17:03:36Z","timestamp":1745946216336},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,10,12]],"date-time":"2020-10-12T00:00:00Z","timestamp":1602460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Activin A belongs to the superfamily of transforming growth factor\u2010\u03b2 and plays an important role in hormone regulation and tissue development. However, few research studies have been conducted on the effect of activin A on feeding organs in fish. In this study, the zebrafish (<jats:italic>Danio rerio<\/jats:italic>) larvae were treated with 1 ng\u2009ml<jats:sup>\u20131<\/jats:sup> activin A for 8\u2009days continuously. The haematoxylin and eosin (H&amp;E) staining section results revealed that the transverse inner diameter of the pharynx and oesophagus significantly increased on the third and eighth days after treatment compared with the control group (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). On the eighth day, the cross\u2010sectional area of the pharyngeal muscle increased by 8638\u2009\u03bcm<jats:sup>2<\/jats:sup> compared to the control group (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). The RNA <jats:italic>in situ<\/jats:italic> hybridization results also showed that the expression of skeletal muscle\u2010specific genes (<jats:italic>myog<\/jats:italic> and <jats:italic>myod<\/jats:italic>) was significantly increased in pharyngeal muscle on the eighth day. Furthermore, the qRT\u2010PCR results showed the expression of <jats:italic>gh<\/jats:italic> gene was significantly increased on the eighth day (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). At the same time, more larvae in activin A group were able to feed larger brine shrimp (<jats:italic>Artemia<\/jats:italic>) than in the control group on the eighth day. In conclusion, activin A could affect feeding by promoting the inner diameter and muscle development of the pharynx and oesophagus in zebrafish larvae. This study is the first to report that the development of the pharynx and oesophagus can directly affect food intake in fish larvae, which provides a theoretical basis for the study of food intake of fish at an early stage.<\/jats:p>","DOI":"10.1111\/jfb.14493","type":"journal-article","created":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T14:06:52Z","timestamp":1597241212000},"page":"1624-1631","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Activin <scp>A<\/scp> affects feeding by promoting the inner diameter and muscle development of the pharynx and oesophagus in zebrafish (<scp><i>Danio rerio<\/i><\/scp>) larvae"],"prefix":"10.1111","volume":"97","author":[{"given":"Shulin","family":"Tang","sequence":"first","affiliation":[{"name":"College of Fisheries, Chinese Perch Research Center Huazhong Agricultural University  Wuhan China"},{"name":"Innovation Base for Chinese Perch Breeding Key Lab of Freshwater Animal Breeding, Ministry of Agriculture  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu\u2010Fang","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Fisheries, Chinese Perch Research Center Huazhong Agricultural University  Wuhan China"},{"name":"Innovation Base for Chinese Perch Breeding Key Lab of Freshwater Animal Breeding, Ministry of Agriculture  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"He","sequence":"additional","affiliation":[{"name":"College of Fisheries, Chinese Perch Research Center Huazhong Agricultural University  Wuhan China"},{"name":"Innovation Base for Chinese Perch Breeding Key Lab of Freshwater Animal Breeding, Ministry of Agriculture  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Shoaib","family":"Alam","sequence":"additional","affiliation":[{"name":"College of Fisheries, Chinese Perch Research Center Huazhong Agricultural University  Wuhan China"},{"name":"Innovation Base for Chinese Perch Breeding Key Lab of Freshwater Animal Breeding, Ministry of Agriculture  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haocan","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Fisheries, Chinese Perch Research Center Huazhong Agricultural University  Wuhan China"},{"name":"Innovation Base for Chinese Perch Breeding Key Lab of Freshwater Animal Breeding, Ministry of Agriculture  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulan","family":"Kuang","sequence":"additional","affiliation":[{"name":"College of Fisheries, Chinese Perch Research Center Huazhong Agricultural University  Wuhan China"},{"name":"Innovation Base for Chinese Perch Breeding Key Lab of Freshwater Animal Breeding, Ministry of Agriculture  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2005.04.023"},{"issue":"1","key":"e_1_2_7_3_1","first-page":"1","article-title":"Morphohistological characteristic of digestive tract of an endemic cichlid fish, Iranocichla hormuzensis Coad, 1982 (Teleostei: Cichlidae)","volume":"8","author":"Ansari M. 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