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The results demonstrated that hepatic lipid metabolism was affected by experimental conditions (diet\/family). The fatty acid composition in the four groups mirrored the differences in dietary composition, but it was also associated with the family groups. Small RNA sequencing followed by RT\u2010qPCR identified 12 differentially expressed microRNAs (DE miRNAs), with expression associated with family groups (miR\u2010146 family members, miR\u2010200b, miR\u2010214, miR\u2010221, miR\u2010125, miR\u2010135, miR\u2010137, miR_nov_1), diets (miR\u2010203, miR\u2010462) or both conditions. All the conserved DE miRNAs have been reported as associated with lipid metabolism in other vertebrates. <jats:italic>In silico<\/jats:italic> predictions revealed 37 lipid metabolism pathway genes, including desaturases, transcription factors and key enzymes in the synthesis pathways as putative targets (e.g., <jats:italic>srebp\u20101<\/jats:italic> and <jats:italic>2<\/jats:italic>, <jats:italic>\u03946fad_b<\/jats:italic> and <jats:italic>c<\/jats:italic>, <jats:italic>hmdh<\/jats:italic>, <jats:italic>elovl4<\/jats:italic> and <jats:italic>5b<\/jats:italic>, <jats:italic>cdc42<\/jats:italic>). RT\u2010qPCR analysis of selected target genes showed expression changes that were associated with diet and with family groups (<jats:italic>d5fad<\/jats:italic>, <jats:italic>d6fad_a<\/jats:italic>, <jats:italic>srebp\u20101<\/jats:italic>). There was a reciprocal difference in the abundance of ssa\u2010miR\u2010203a\u20103p and <jats:italic>srebp\u20101<\/jats:italic> in one group comparison, whereas other predicted targets did not reveal any evidence of being negatively regulated by degradation. More experimental studies are needed to validate and fully understand the predicted interactions and how the DE miRNAs may participate in the regulation of hepatic lipid metabolism.<\/jats:p>","DOI":"10.1111\/jfb.14649","type":"journal-article","created":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T09:28:23Z","timestamp":1608283703000},"page":"1172-1185","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Modulation of hepatic <scp>miRNA<\/scp> expression in Atlantic salmon (<scp><i>Salmo salar<\/i><\/scp>) by family background and dietary fatty acid composition"],"prefix":"10.1111","volume":"98","author":[{"given":"Tone\u2010Kari K.","family":"\u00d8stbye","sequence":"first","affiliation":[{"name":"Nofima (Norwegian Institute of Food, Fisheries, and Aquaculture Research)  \u00c5s Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nardos T.","family":"Woldemariam","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Health, Faculty of Health Sciences OsloMet \u2013 Oslo Metropolitan University  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camilla E.","family":"Lundberg","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Health, Faculty of Health Sciences OsloMet \u2013 Oslo Metropolitan University  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerd M.","family":"Berge","sequence":"additional","affiliation":[{"name":"Nofima (Norwegian Institute of Food, Fisheries, and Aquaculture Research)  \u00c5s Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bente","family":"Ruyter","sequence":"additional","affiliation":[{"name":"Nofima (Norwegian Institute of Food, Fisheries, and Aquaculture Research)  \u00c5s Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8773-1273","authenticated-orcid":false,"given":"Rune","family":"Andreassen","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Health, Faculty of Health Sciences OsloMet \u2013 Oslo Metropolitan University  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201302647"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-10-r106"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2017.02.023"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153324"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-017-3741-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-482"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2015.25"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/132.2.222"},{"key":"e_1_2_7_10_1","first-page":"1","volume-title":"Betydning av genetisk bakgrunn og ulike niv\u00e5er av omega\u20103\u2010fettsyrer i f\u00f4r i tidlige livsfaser for fiskehelse, fettsyresammensetning og muskelkvalitet ved slaktest\u00f8rrelse","author":"Berge G. 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