{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:46:26Z","timestamp":1792554386167,"version":"4.5.0"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,2,17]],"date-time":"2021-02-17T00:00:00Z","timestamp":1613520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Natural history collections are repositories of biodiversity and are potentially used by molecular ecologists for comparative taxonomic, phylogenetic, biogeographic and forensic purposes. Specimens in fish collections are preserved using a combination of methods with many fixed in formalin and then preserved in ethanol for long\u2010term storage. Formalin fixation damages DNA, thereby limiting genetic analyses. In this study, the authors compared the DNA barcoding and identification success for frozen and formalin\u2010fixed tissues obtained from specimens in the CSIRO Australian National Fish Collection. They studied 230 samples from fishes (consisting of &gt;160 fish species). An optimized formalin\u2010fixed, paraffin\u2010embedded DNA extraction method resulted in usable DNA from degraded tissues. Four mini barcoding assays of the mitochondrial DNA (mtDNA) were characterized with Sanger and Illumina amplicon sequencing. In the good quality DNA (without exposure to formalin), up to 88% of the specimens were correctly matched at the species level using the cytochrome oxidase subunit 1 (COI) mini barcodes, whereas up to 58% of the specimens exposed to formalin for less than 8\u2009weeks were correctly identified to species. In contrast, 16S primers provided higher amplification success with formalin\u2010exposed tissues, although the COI gene was more successful for identification. Importantly, the authors found that DNA of a certain size and quality can be amplified and sequenced despite exposure to formalin, and Illumina sequencing provided them with greater power of resolution for taxa identification even when there was little DNA present. Overall, within parameter constraints, this study highlights the possibilities of recovering DNA barcodes for identification from formalin\u2010fixed fish specimens, and the authors provide guidelines for when successful identification could be expected.<\/jats:p>","DOI":"10.1111\/jfb.14687","type":"journal-article","created":{"date-parts":[[2021,1,23]],"date-time":"2021-01-23T05:29:33Z","timestamp":1611379773000},"page":"1421-1432","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Assessing\n                    <scp>DNA<\/scp>\n                    for fish identifications from reference collections: the good, bad and ugly shed light on formalin fixation and sequencing approaches"],"prefix":"10.1111","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3105-1690","authenticated-orcid":false,"given":"Sharon A.","family":"Appleyard","sequence":"first","affiliation":[{"name":"CSIRO Australian National Fish Collection National Research Collections Australia  Hobart Tasmania Australia"},{"name":"CSIRO Environomics Future Science Platform  Canberra Australian Capital Territory Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7874-1875","authenticated-orcid":false,"given":"Safia","family":"Maher","sequence":"additional","affiliation":[{"name":"CSIRO Australian National Fish Collection National Research Collections Australia  Hobart Tasmania Australia"},{"name":"CSIRO Environomics Future Science Platform  Canberra Australian Capital Territory Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4878-3919","authenticated-orcid":false,"given":"John J.","family":"Pogonoski","sequence":"additional","affiliation":[{"name":"CSIRO Australian National Fish Collection National Research Collections Australia  Hobart Tasmania Australia"},{"name":"CSIRO Environomics Future Science Platform  Canberra Australian Capital Territory Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1563-2855","authenticated-orcid":false,"given":"Stephen J.","family":"Bent","sequence":"additional","affiliation":[{"name":"CSIRO Environomics Future Science Platform  Canberra Australian Capital Territory Australia"},{"name":"Data 61, CSIRO  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3036-1873","authenticated-orcid":false,"given":"Xin\u2010Yi","family":"Chua","sequence":"additional","affiliation":[{"name":"CSIRO Environomics Future Science Platform  Canberra Australian Capital Territory Australia"},{"name":"Data 61, CSIRO  Brisbane Queensland Australia"},{"name":"School of Electrical Engineering and Computer Science Queensland University of Technology  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1765-7039","authenticated-orcid":false,"given":"Annette","family":"McGrath","sequence":"additional","affiliation":[{"name":"CSIRO Environomics Future Science Platform  Canberra Australian Capital Territory Australia"},{"name":"Data 61, CSIRO  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-9993.2001.01070.pp.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1139\/f01-004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/00949650215733"},{"key":"e_1_2_8_5_1","unstructured":"Andrews S.(2015). 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