{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:27:13Z","timestamp":1747186033684,"version":"3.40.5"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T00:00:00Z","timestamp":1659916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study was carried out to study the larval skeletal development in <jats:italic>Labeo calbasu<\/jats:italic> by using a modified double skeletal staining technique with Alizarin red and Alcian blue. The larval samples were obtained after induced breeding of wild <jats:italic>L. calbasu<\/jats:italic> germplasm from the River Ganga. Samples from 2\u00a0to 20\u2009dph (day post hatching) were preserved in 4% neutral phosphate buffered formalin solution. Alizarin red and Alcian blue were used to stain the bony and cartilage parts of the skeleton, respectively. The size of the specimens ranged from 6.6\u00a0\u00b1\u20090.16 to 15.6\u00a0\u00b1\u20091.15\u2009mm. The development of skeleton was observed at very early stages. A straight notochord throughout its length and origin of caudal fin rays were seen on 2\u00a0dph. The ventral spines, unbranched caudal fin rays and hypurals at ventral side of notochord were clearly visible from 4\u00a0dph. Most of the head skeletal elements and vertebral column with vertebral centrum and neural spines started appearing at 4\u2009dph. The dorsal and caudal fins with branched rays and the opercular and jaw bones started ossifying between 10 and 20\u2009dph. The present study gives an idea about the skeletal development process as well as detects the skeletal abnormalities in Indian major carp, <jats:italic>L. calbasu<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jfb.15143","type":"journal-article","created":{"date-parts":[[2022,6,25]],"date-time":"2022-06-25T04:09:30Z","timestamp":1656130170000},"page":"834-845","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ontogeny of skeletal ossification in <i>Labeo calbasu<\/i> (Hamilton, 1822) using differential fluorescence staining technique"],"prefix":"10.1111","volume":"101","author":[{"given":"Himanshu S.","family":"Swain","sequence":"first","affiliation":[{"name":"ICAR\u2010Central Inland Fisheries Research Institute  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ambily","family":"MN","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Inland Fisheries Research Institute  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amir K.","family":"Samal","sequence":"additional","affiliation":[{"name":"ICAR\u2010Indian Veterinary Research Institute  Bareilly India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitesh H.","family":"Ramteke","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Inland Fisheries Research Institute  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6629-8992","authenticated-orcid":false,"given":"Basanta K.","family":"Das","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Inland Fisheries Research Institute  Kolkata India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,8]]},"reference":[{"issue":"2","key":"e_1_2_8_2_1","article-title":"Modified double skeletal staining protocols with Alizarinred and Alcian blue in laboratory animals","volume":"13","author":"Aliesfehani T.","year":"2015","journal-title":"Annals of Military & Health Sciences Research"},{"volume-title":"Standard methods for the examination of water and wastewater","year":"2012","author":"American Public Health Association","key":"e_1_2_8_3_1"},{"volume-title":"Handbook of fisheries and aquaculture","year":"2006","author":"Ayyappan S.","key":"e_1_2_8_4_1"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-010-9258-6"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12861-016-0102-4"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10228-014-0413-z"},{"issue":"2","key":"e_1_2_8_8_1","first-page":"249","article-title":"Ecological assessment of fishes and population dynamics of Labeo rohita (Hamilton), tor tor (Hamilton) and Labeo calbasu (Hamilton) in the Paisuni river","volume":"10","author":"Dwivedi A. 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