{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T21:33:53Z","timestamp":1779917633546,"version":"3.53.1"},"reference-count":62,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,6,18]],"date-time":"2023-06-18T00:00:00Z","timestamp":1687046400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aldehyde reductase (ALR) plays key roles in the detoxification of toxic aldehyde. In this study, the authors cloned the swamp eel ALR gene using rapid amplification of cDNA ends\u2010PCR (RACE\u2010PCR). The recombinant protein (rALR) was expressed in <jats:italic>Escherichia coli<\/jats:italic> and purified using a Ni<jats:sup>2+<\/jats:sup>\u2010NTA chelating column. The rALR protein exhibited efficient reductive activity towards several aldehydes, ketones and S\u2010nitrosoglutathione (GSNO). A spot assay suggested that the recombinant <jats:italic>E. coli<\/jats:italic> strain expressing rALR showed better resistance to formaldehyde, sodium nitrite and GSNO stress, suggesting that swamp eel ALR is crucial for redox homeostasis <jats:italic>in vivo<\/jats:italic>. Consequently, the authors investigated the effect of rALR on the oxidative parameters of the liver in swamp eels challenged with <jats:italic>Aeromonas hydrophila<\/jats:italic>. The hepatic glutathione (GSH) content significantly increased, and the hepatic NO content and levels of reactive oxygen species and reactive nitrogen species significantly decreased when rALR was administered. In addition, the mRNA expression of hepatic <jats:italic>Alr<\/jats:italic>, <jats:italic>HO1<\/jats:italic> and <jats:italic>Nrf2<\/jats:italic> was significantly upregulated, whereas the expression levels of <jats:italic>NF\u2010\u03baB<\/jats:italic>, <jats:italic>IL\u20101\u03b2<\/jats:italic> and <jats:italic>NOS1<\/jats:italic> were significantly downregulated in the rALR\u2010administered group. Collectively, these results suggest that ALR is involved in the response to nitrosative stress by regulating GSH\/NO levels in the swamp eel.<\/jats:p>","DOI":"10.1111\/jfb.15471","type":"journal-article","created":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T15:29:11Z","timestamp":1685719751000},"page":"529-543","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Swamp eel aldehyde reductase is involved in response to nitrosative stress <i>via<\/i> regulating <scp>NO<\/scp>\/<scp>GSH<\/scp> levels"],"prefix":"10.1111","volume":"103","author":[{"given":"Yuhe","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Life Sciences Yangtze University  Jingzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Sciences Yangtze University  Jingzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfen","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Life Sciences Yangtze University  Jingzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Kong","sequence":"additional","affiliation":[{"name":"College of Life Sciences Yangtze University  Jingzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sifan","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Life Sciences Yangtze University  Jingzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guopan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences Yangtze University  Jingzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1792-5336","authenticated-orcid":false,"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences Yangtze University  Jingzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-011-1536-9"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(00)00293-8"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20140654"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1001-907"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)60566-6"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.22731.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.25.5.4487"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2021.07.015"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2021.09.007"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13578-020-00432-0"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0173974"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2011.04.005"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2016.00206"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erv099"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10189"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(99)00060-X"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.44.101802.121844"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1800431115"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1004"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-1965-2_25"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.12.10.773"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehr304"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo11060343"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2018.09.018"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJGM.S15618"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/15376516.2016.1238529"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/adtp.202100032"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.16.11929"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2009.02.002"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2012.12.026"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.108.022251"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(01)00032-2"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2000.01601.x"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.14458"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12686-011-9549-6"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2020.09.004"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.101763"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.06.036"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/35068596"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-016-2223-0"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2017.01.003"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2012.09.008"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1080\/10715762.2018.1457217"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)35292-4"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3430487"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3109\/15376516.2012.666684"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2010.12.003"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrestox.6b00319"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2022.100419"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2017.11.005"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-061516-045037"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.011067"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90728-L"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2017-0152"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-28242-7"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1524"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2013.05.012"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10695-022-01048-6"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22189794"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-014-0164-8"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10695-020-00846-0"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0749-z"}],"container-title":["Journal of Fish Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfb.15471","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T06:29:06Z","timestamp":1750314546000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfb.15471"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,18]]},"references-count":62,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["10.1111\/jfb.15471"],"URL":"https:\/\/doi.org\/10.1111\/jfb.15471","archive":["Portico"],"relation":{},"ISSN":["0022-1112","1095-8649"],"issn-type":[{"value":"0022-1112","type":"print"},{"value":"1095-8649","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,18]]},"assertion":[{"value":"2023-03-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}