{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:26:18Z","timestamp":1790767578848,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,6,22]],"date-time":"2023-06-22T00:00:00Z","timestamp":1687392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The andrological study of a species involves the macro\u2010 and microscopic analyses of the internal reproductive organs and the evaluation of seminal parameters and ultrastructural characteristics of the spermatozoa. As in other vertebrates, the male reproductive tract in chondrichthyans consists of testes and reproductive ducts (efferent duct, epididymis, Leydig's gland, ductus deferens and seminal vesicle). In this study the authors used three adult specimens of <jats:italic>Zapteryx brevirostris<\/jats:italic> from wild capture kept at the Ubatuba Aquarium, Brazil. Semen was collected by abdominal massage over the location of the seminal vesicle, preceded by ultrasonographic evaluation. The semen collected was diluted 1:200 and subject to quantitative and morphological analyses. Ultrastructural analysis was performed using transmission and scanning electron microscopy. Correlation was observed between successful collection and ultrasonographic image of an engorged seminal vesicle, as well as testicles with easily delimitable margins and higher echogenicity. It was possible to identify free spermatozoa with helical filiform appearance, as well as spermatozeugmata. The average sperm concentration resulted in 5\u2009million packets per millilitre and 140\u2009million spermatozoa per millilitre. The sperm nucleus is described as follows: cone shaped, parachromatin sheath less dense than the chromatin of the nucleus, smooth depression of the nuclear fossa, abaxial axoneme 9\u2009+\u20092 and accessory axonemal columns in positions 3 and 8 and oval shaped, with flattened inner surface in cross\u2010section. These results broaden the knowledge of the andrology of this species, contributing to <jats:italic>ex situ<\/jats:italic> breeding programmes.<\/jats:p>","DOI":"10.1111\/jfb.15479","type":"journal-article","created":{"date-parts":[[2023,6,10]],"date-time":"2023-06-10T03:09:35Z","timestamp":1686366575000},"page":"635-645","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Andrology of shortnose guitarfish <i>Zapteryx brevirostris<\/i> (M\u00fcller &amp; Henle, 1841) (Chondrichthyes, Trygonorrhinidae)"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9228-970X","authenticated-orcid":false,"given":"Laura","family":"de Oliveira Camilo","sequence":"first","affiliation":[{"name":"Ubatuba Aquarium  Ubatuba Brazil"},{"name":"Marine Fish Laboratory Fisheries Institute  Ubatuba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9064-8596","authenticated-orcid":false,"given":"Bruna Larissa","family":"Maganhe","sequence":"additional","affiliation":[{"name":"Ubatuba Aquarium  Ubatuba Brazil"},{"name":"Marine Fish Laboratory Fisheries Institute  Ubatuba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7769-5638","authenticated-orcid":false,"given":"Hugo","family":"Gallo Neto","sequence":"additional","affiliation":[{"name":"Ubatuba Aquarium  Ubatuba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7273-6381","authenticated-orcid":false,"given":"Silvia Edelweiss","family":"Crusco","sequence":"additional","affiliation":[{"name":"Universidade Paulista  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5825-9259","authenticated-orcid":false,"given":"Carlos Eduardo","family":"Malavasi\u2010Bruno","sequence":"additional","affiliation":[{"name":"Laboratory of Fish and Aquatic Health Fluminense Federal University  Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9976-9271","authenticated-orcid":false,"given":"Eduardo Gomes","family":"Sanches","sequence":"additional","affiliation":[{"name":"Marine Fish Laboratory Fisheries Institute  Ubatuba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.20647"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1679-62252011005000014"},{"key":"e_1_2_8_4_1","first-page":"148","article-title":"Histology of the testicles and male reproductive tract of the skates Sympterygia acuta (Garman, 1877) and S. bonapartii (M\u00fcller & Henle, 1841) (Chondrichthyes: Rajoidei) in the Western South Atlantic Ocean","volume":"13","author":"Basallo A.","year":"2018","journal-title":"Pan\u2010American Journal of Aquatic Sciences"},{"key":"e_1_2_8_5_1","first-page":"301","article-title":"Desenvolvimento sexual de Zapteryx brevirostris (M\u00fcller & Henle, 1841) no litoral do Rio de Janeiro, Brasil","volume":"47","author":"Batista V. 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