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For this reason, alkaline proteinases from viscera of Argentine hake <jats:italic>Merluccius hubbsi<\/jats:italic>, Brazilian flathead <jats:italic>Percophis brasiliensis<\/jats:italic>, Brazilian codling <jats:italic>Urophycis brasiliensis<\/jats:italic>, and stripped weakfish <jats:italic>Cynoscion guatucupa<\/jats:italic> were characterized. Individuals were caught by a commercial fleet off the coast of the Argentinean Sea. The intestine and pyloric caeca were dissected out and then minced and triturated with distilled water. The proteinase activity of <jats:italic>P. brasiliensis<\/jats:italic> extracts was enhanced by all the ions tested (Mn<jats:sup>2+<\/jats:sup>, K<jats:sup>+<\/jats:sup>, Na<jats:sup>+<\/jats:sup>, Ca<jats:sup>+2<\/jats:sup>) while the enzymes of the other species were stable in the presence of those ions, retaining more than 60% of their enzymatic activity. Alkaline proteinases of all species showed extreme stability to 5% v\/v surfactants at 60\u2009min (Sodium dodecyl sulfate, Triton X\u2010100, Tween 20, Tween 80), and relative stability toward an 6% v\/v oxidizing agent (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) and organic solvents 80% (acetone, isopropanol, methanol, ethanol). The enzyme extracts were incubated for 60\u2009min with these compounds. Interestingly, alkaline proteinases from all species were compatible with the commercial detergents (Ala, Skip, and Ace). These results demonstrate that proteinases recovered from a no\u2010cost sample such as fishery residues can be used for industrial applications, such as detergent formulations.<\/jats:p>","DOI":"10.1111\/jfb.16038","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T06:18:41Z","timestamp":1734934721000},"page":"1183-1194","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Recovery of alkaline proteinases from fisheries wastes: biochemical characterization and applications"],"prefix":"10.1111","volume":"106","author":[{"given":"Ivana Soledad","family":"Friedman","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales Universidad Nacional de Mar del Plata, CCT \u2013 Mar del Plata, Consejo Nacional de Investigaciones Cient\u00edficas y Tecnicas  Mar del Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgardo Mart\u00edn","family":"Contreras","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Ciencia y Tecnolog\u00eda de Materiales, CCT \u2013 Mar del Plata Consejo Nacional de Investigaciones Cient\u00edficas y Tecnicas  Mar del Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9232-4560","authenticated-orcid":false,"given":"Analia Ver\u00f3nica","family":"Fern\u00e1ndez\u2010Gimenez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales Universidad Nacional de Mar del Plata, CCT \u2013 Mar del Plata, Consejo Nacional de Investigaciones Cient\u00edficas y Tecnicas  Mar del Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2019.09.010"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcatb.2005.11.011"},{"issue":"6","key":"e_1_2_8_4_1","first-page":"61","article-title":"Criterios de selecci\u00f3n de cepas f\u00fangicas para la producci\u00f3n de proteasas termoestables por cultivo en medio s\u00f3lido","volume":"1","author":"Barragan L. 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