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Considering the increasing scientific interest for TNF\u2010\u03b1 in piscine biology, the paper detailed the characteristics of a novel <jats:italic>tnf\u2010\u03b1<\/jats:italic> gene from a high\u2010value mariculture species, <jats:italic>Trachinotus blochii<\/jats:italic>. The <jats:italic>tbtnf<\/jats:italic>\u2010\u03b1 complementary DNA (cDNA) of 1385\u2009bp encoded an open reading frame of 762\u2009bp, 3\u2032 untranslated region (3\u2032 UTR) of 484\u2009bp and 5\u2032 UTR of 139\u2009bp. The deduced Tbtnf\u2010\u03b11 showed the highest sequence identity to that of <jats:italic>Seriola dumerili<\/jats:italic> (\u223c83%). The comparative phylogenetics identified the protein as the TNF\u2010\u03b1 paralog 1. Tbtnf\u2010\u03b11 displayed all the hallmark features of other teleost TNF\u2010\u03b1, suggesting similar immune\u2010related functions. However, the Tbtnf\u2010\u03b11 was predicted to be more acidic and less thermostable. The study generated the three\u2010dimensional (3\u2010D) structure model of Tbtnf\u03b1\u20101 based on the protein sequence that can be applied in future research. The genomic organization of <jats:italic>tbtnf\u03b1\u20101<\/jats:italic> contained four exons and three introns. Real\u2010time polymerase chain reaction (PCR) analysis in healthy conditions showed the constitutive expression and wide distribution of <jats:italic>tbtnf\u03b1\u20101<\/jats:italic> in a tissue\u2010specific manner, with maximum expression in the kidney. As in silico analysis predicted the biological function of the novel TNF\u2010\u03b1 as a regulator in the defence response to bacterial infection, functional validation was done through the expression analysis following exposure to a marine pathogen (<jats:italic>Vibrio harveyi)<\/jats:italic> challenge. The results showed that the expression of <jats:italic>tbtnf\u2010\u03b11<\/jats:italic> in the liver, spleen, heart, gill and kidney was significantly upregulated compared to the control fish after the challenge at differential time points post\u2010infection. The study also demonstrated the kinetics of <jats:italic>tbtnf\u03b1\u20101<\/jats:italic> expression in the liver, spleen, heart, gill and kidney at 6 to 48\u2009h post\u2010infection, and the results showed the peak expression at 24\u2009h in all the tissues followed by a drop in the expression. Briefly, the present study detailed the sequence, structural and functional characteristics of <jats:italic>tnf\u2010\u03b11<\/jats:italic> in the immune response of silver pompano.<\/jats:p>","DOI":"10.1111\/jfb.70077","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T03:19:47Z","timestamp":1747797587000},"page":"808-823","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular features and expression characteristics of a novel tumour necrosis factor\u2010\u03b1 paralog from snubnose pompano (<i>Trachinotus blochii<\/i>)"],"prefix":"10.1111","volume":"107","author":[{"given":"Jagannivasan","family":"Amritha","sequence":"first","affiliation":[{"name":"Marine Biotechnology, Fish Nutrition and Health Division ICAR\u2010Central Marine Fisheries Research Institute  Kochi India"},{"name":"Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences Cochin University of Science and Technology  Kochi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thangalazhy Gopakumar","family":"Sumithra","sequence":"additional","affiliation":[{"name":"Marine Biotechnology, Fish Nutrition and Health Division ICAR\u2010Central Marine Fisheries Research Institute  Kochi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3342-7716","authenticated-orcid":false,"given":"Sulumane Ramachandra","family":"Krupesha Sharma","sequence":"additional","affiliation":[{"name":"Marine Biotechnology, Fish Nutrition and Health Division ICAR\u2010Central Marine Fisheries Research Institute  Kochi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Velappan Nair","family":"Anusree","sequence":"additional","affiliation":[{"name":"Marine Biotechnology, Fish Nutrition and Health Division ICAR\u2010Central Marine Fisheries Research Institute  Kochi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikathil Raveendranathan","family":"Dhanutha","sequence":"additional","affiliation":[{"name":"Marine Biotechnology, Fish Nutrition and Health Division ICAR\u2010Central Marine Fisheries Research Institute  Kochi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Reynold","sequence":"additional","affiliation":[{"name":"Marine Biotechnology, Fish Nutrition and Health Division ICAR\u2010Central Marine Fisheries Research Institute  Kochi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph Chakkalakkal","family":"George","sequence":"additional","affiliation":[{"name":"Marine Biotechnology, Fish Nutrition and Health Division ICAR\u2010Central Marine Fisheries Research Institute  Kochi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh","family":"Gayathri","sequence":"additional","affiliation":[{"name":"Marine Biotechnology, Fish Nutrition and Health Division ICAR\u2010Central Marine Fisheries Research Institute  Kochi India"},{"name":"Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences Cochin University of Science and Technology  Kochi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Purackattu Gop","family":"Ambarish","sequence":"additional","affiliation":[{"name":"Mariculture Division Vizhinjam Regional Centre of ICAR\u2010Central Marine Fisheries Research Institute  Thiruvananthapuram India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achamveetil","family":"Gopalakrishnan","sequence":"additional","affiliation":[{"name":"Marine Biotechnology, Fish Nutrition and Health Division ICAR\u2010Central Marine Fisheries Research Institute  Kochi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00214-2"},{"key":"e_1_2_10_3_1","first-page":"15","article-title":"CFSSP: Chou and Fasman secondary structure prediction server","volume":"1","author":"Ashok Kumar T.","year":"2013","journal-title":"Wide Spectrum"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4657"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2013.06.004"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.6.1670"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1515\/pjvs-2017-0071"},{"key":"e_1_2_10_8_1","first-page":"444","article-title":"Recent developments in RACE\u2010PCR for the full length cDNA identification","volume":"8","author":"Chutia S. 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