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This study aims to understand the feeding behaviours of the roosterfish by analysing the stomach content (SCA) and performing bulk stable isotope analysis (BSIA). The study also seeks to identify ontogenetic changes in its feeding habits and habitat preferences. To achieve this, 505 stomachs and 165 muscle samples were analysed in three locations in Baja California Sur (Cabo San Lucas, La Paz and Puerto San Carlos) between 2010 and 2017. The SCA determined that the diet consisted mainly of fish from the families Carangidae, Engraulidae and Clupeidae, which together constituted almost 50% of the total diet. Statistical differences in diet composition were found by size class but not by sex or the interaction between these variables. Concerning BSIA, there were no significant differences between males and females; however, differences were observed in the \u2018undifferentiated sex\u2019 grouping, which was comprised largely small juvenile individuals. Concerning size, BSIA values were correlated with roosterfish fork length, so the observed differences for the \u2018undifferentiated sex\u2019 category were related to size variation rather than sexual maturity. The findings from this study suggest that roosterfish experience an ontogenetic increase in trophic position, relying less on sardine\u2010like species as they grow. Furthermore, BSIA values could inform on relative residency of the roosterfish to the Baja California peninsula.<\/jats:p>","DOI":"10.1111\/jfb.70079","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T01:24:03Z","timestamp":1747272243000},"page":"776-787","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Trophic ecology of roosterfish (<scp><i>Nematistius pectoralis<\/i><\/scp>) inferred from stomach content and stable isotope analyses in the southern part of Baja California peninsula"],"prefix":"10.1111","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2257-3534","authenticated-orcid":false,"given":"Sof\u00eda","family":"Ortega\u2010Garc\u00eda","sequence":"first","affiliation":[{"name":"Departamento de Pesquer\u00edas y Biolog\u00eda Marina Instituto Polit\u00e9cnico Nacional\u2010Centro Interdisciplinario de Ciencias Marinas, Av. 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