{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:08:01Z","timestamp":1792033681487,"version":"4.4.0"},"reference-count":85,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T00:00:00Z","timestamp":1755561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100013288","name":"LIFE programme","doi-asserted-by":"publisher","award":["Project 101074458 \u2010 LIFE21\u2010NAT\u2010IT\u2010PREDATOR"],"award-info":[{"award-number":["Project 101074458 \u2010 LIFE21\u2010NAT\u2010IT\u2010PREDATOR"]}],"id":[{"id":"10.13039\/100013288","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Morphological and metabarcoding analyses provided new insights into the diet of the European catfish (\n                    <jats:italic>Silurus glanis<\/jats:italic>\n                    ) in an invaded area in the Iberian Peninsula. Out of 264\n                    <jats:italic>S. glanis<\/jats:italic>\n                    examined, data were obtained for 173 individuals through metabarcoding of intestinal contents and for 110 through morphological analysis of stomach contents. Metabarcoding consistently identified more prey taxa per sample than morphological analysis, yielding a total of 34 and 21 taxa, respectively, that primarily included fish and crustaceans. Morphological analysis demonstrated a higher detection of crustaceans than metabarcoding, which detected more fish. Both methods identified native\n                    <jats:italic>Anguilla anguilla, Chelon<\/jats:italic>\n                    spp.,\n                    <jats:italic>Luciobarbus<\/jats:italic>\n                    spp. and\n                    <jats:italic>Atyaephyra desmarestii<\/jats:italic>\n                    as well as\u00a0non\u2010native\n                    <jats:italic>Lepomis gibbosus<\/jats:italic>\n                    as the most frequent prey. However, metabarcoding detected\n                    <jats:italic>A. anguilla<\/jats:italic>\n                    at more than twice the frequency found by morphological analysis, and other endangered taxa that were missed by morphological analysis. Metabarcoding showed that prey composition was influenced by both season and predator total length, suggesting dietary shifts related to prey availability and foraging habits. Taken together, results indicate that metabarcoding may be important for assessing the full range of prey diversity and uncover patterns in prey use, particularly fish, providing more information from fewer samples compared to morphological analysis and can contribute to conservation management. Metabarcoding results suggest that predation by the\n                    <jats:italic>S. glanis<\/jats:italic>\n                    on endangered and economically valuable fish such as\n                    <jats:italic>A. anguilla<\/jats:italic>\n                    ,\n                    <jats:italic>Alosa<\/jats:italic>\n                    spp. may be stronger than previously considered.These findings reinforce the urgent need for targeted management actions to mitigate the impacts of\n                    <jats:italic>S. glanis<\/jats:italic>\n                    in the Iberian Peninsula, where many native and endemic species are currently threatened.\n                  <\/jats:p>","DOI":"10.1111\/jfb.70152","type":"journal-article","created":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T05:05:42Z","timestamp":1755666342000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Hidden in the gut: Metabarcoding reveals overlooked predation by the invasive European catfish (\n                    <scp>\n                      <i>Silurus glanis<\/i>\n                    <\/scp>\n                    )"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5042-8599","authenticated-orcid":false,"given":"Mafalda","family":"Moncada","sequence":"first","affiliation":[{"name":"MARE, Marine and Environmental Sciences Centre\/ARNET, Aquatic Research Network Faculty of Sciences, University of Lisbon  Lisbon Portugal"},{"name":"Faculty of Sciences, University of Lisbon  Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6654-5300","authenticated-orcid":false,"given":"Sofia","family":"Nogueira","sequence":"additional","affiliation":[{"name":"MARE, Marine and Environmental Sciences Centre\/ARNET, Aquatic Research Network Faculty of Sciences, University of Lisbon  Lisbon 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