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The Antarctic icefish, which lack haemoglobin and functional red blood cells, serves as a unique model to explore the impact of acetylation on haematopoiesis and cold\u2010adaptive epigenetic mechanisms. Despite its relevance, the specific acetylation mechanisms in Antarctic fish remain poorly understood. In this study, we utilized a comprehensive methodology combining tandem mass tag labelling, high\u2010performance liquid chromatography fractionation, Kac antibody affinity enrichment and liquid chromatography\u2013tandem mass spectrometry to quantify and compare acetylation patterns in the haematopoietic tissues of the head kidney between a white\u2010blooded icefish\n                    <jats:italic>Chionodraco hamatus<\/jats:italic>\n                    and a red\u2010blooded species\n                    <jats:italic>Trematomus bernacchii<\/jats:italic>\n                    . A total of 167 acetylation sites on lysine residues were identified across 77 differentially acetylated proteins, including 10 proteins with upregulated acetylation and eight proteins with downregulated acetylation between the two species. Kyoto Encyclopedia of Genes and Genomes pathway analysis of these proteins indicated significant enrichment in the \u2018Cell proliferation and cell cycle\u2019, \u2018P53 signaling pathway\u2019, and \u2018FoxO signaling pathway\u2019 for the upregulated acetylated proteins in the icefish head kidney, while \u2018Systemic lupus erythematosus pathways\u2019 and \u2018Gene regulation pathways\u2019 were found in the downregulated proteins. Furthermore, a marked reduction in acetylation of the histone H2B type 1\u2010M\u2010like (H2B) protein was observed in the icefish head kidney, potentially reflecting the species' extremely low red blood cell production. This study represents the first detailed exploration of protein acetylation in Antarctic fish and provides valuable insights into the epigenetic adaptations of marine vertebrates to cold environments.\n                  <\/jats:p>","DOI":"10.1111\/jfb.70203","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T05:06:42Z","timestamp":1758604002000},"page":"243-257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative analysis of differentially acetylated proteins between Antarctic white\u2010blooded\n                    <scp>\n                      <i>Chionodraco hamatus<\/i>\n                    <\/scp>\n                    and red\u2010blooded\n                    <scp>\n                      <i>Trematomus bernacchii<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"108","author":[{"given":"Ruonan","family":"Jia","sequence":"first","affiliation":[{"name":"Key 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