{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T20:18:34Z","timestamp":1789589914167,"version":"build-2803163510"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T00:00:00Z","timestamp":1758326400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T00:00:00Z","timestamp":1758326400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The saccus vasculosus (SV) is a specialized periventricular organ found in some species of teleost and elasmobranch fishes, located at the back of the hypothalamus and pituitary gland. However, its function remains incompletely understood. In this study, histological, transmission electron microscopy (TEM) and transcriptomic analyses were employed to investigate the SV of the mandarin fish (\n                    <jats:italic>Siniperca chuatsi<\/jats:italic>\n                    ) to gain insights into its morphology, gene expression profile and function. Through histological staining techniques, different cell types within the SV can be identified and characterized. The SV is highly folded vascularized neuroepithelium and is deeply connected to blood vessels. TEM studies showed that the capillaries that produced the lumen formed complex narrow channels are linked to the third ventricle of the hypothalamus. The bright neuroepithelium is formed by coronet cells and supporting glial cells, interspersed with liquor\u2010containing neurons, while the dark contrast is attributed to the presence of heavy elements such as calcium. We identified 28 specific genes in SV, which are most enriched in the purine metabolism pathway, including the genes of\n                    <jats:italic>pde9al<\/jats:italic>\n                    ,\n                    <jats:italic>pde9ac<\/jats:italic>\n                    ,\n                    <jats:italic>pde6hl<\/jats:italic>\n                    and\n                    <jats:italic>sgc88el<\/jats:italic>\n                    . Gene Ontology biological process\u00a0analysis for the SV\u2010specific genes showed that response to osmotic stress was the hub term according to the enrichment maps. Additionally, SV\u2010specific genes opsin (\n                    <jats:italic>opsin8b<\/jats:italic>\n                    ), photoreceptor (\n                    <jats:italic>pcare1<\/jats:italic>\n                    ) and aquaporin (\n                    <jats:italic>aqp4<\/jats:italic>\n                    ,\n                    <jats:italic>aqp9l<\/jats:italic>\n                    ) of\n                    <jats:italic>Siniperca chuatsi<\/jats:italic>\n                    may indicate that they play a role as a sensor of season and are involved in osmoregulation. This study, the first on the\n                    <jats:italic>Siniperca chuatsi<\/jats:italic>\n                    , suggests that the SV may have unique physiological functions in teleost fish.\n                  <\/jats:p>","DOI":"10.1111\/jfb.70232","type":"journal-article","created":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T11:35:22Z","timestamp":1758368122000},"page":"232-242","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Morphological and functional analysis of saccus vasculosus of mandarin fish (\n                    <scp>\n                      <i>Siniperca chuatsi<\/i>\n                    <\/scp>\n                    )"],"prefix":"10.1111","volume":"108","author":[{"given":"Rahma Sakina Said","family":"Aly","sequence":"first","affiliation":[{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajie","family":"Fang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keying","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Fisheries Henan Normal University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiong","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Fisheries Henan Normal University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Fisheries Henan Normal University  Xinxiang China"},{"name":"Observation and Research Station on Water Ecosystem in Danjiangkou Reservoir of Henan Province  Nanyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowu","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai China"},{"name":"Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2595-7995","authenticated-orcid":false,"given":"Yawei","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Fisheries Henan Normal University  Xinxiang China"},{"name":"Observation and Research Station on Water Ecosystem in Danjiangkou Reservoir of Henan Province  Nanyang China"},{"name":"Department of Biological Sciences Clemson University  Clemson South Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,20]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"293","article-title":"The saccus vasculosus","volume":"5","author":"Altner H.","year":"1972","journal-title":"The structure and function of nervous tissue"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00307255"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000144902"},{"key":"e_1_2_8_5_1","first-page":"186","article-title":"The saccus vasculosus of fishes, a receptive nervous organ and not a gland","volume":"13","author":"Boeke J.","year":"1910","journal-title":"Amsterdam Proceedings Science Koninklijke Akademie Van Wetenschappen"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2022.12.006"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15897"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani9050233"},{"key":"e_1_2_8_9_1","first-page":"151","article-title":"Light, scanning and transmission electron microscopy study of fish cerebellar capillaries","volume":"1","author":"Castej\u00f3n O. 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