{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T17:14:18Z","timestamp":1775495658059,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD2400401"],"award-info":[{"award-number":["2022YFD2400401"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2022QC042"],"award-info":[{"award-number":["ZR2022QC042"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Turbot (\n                    <jats:italic>Scophthalmus maximus<\/jats:italic>\n                    ) is one of the most important marine aquaculture species worldwide. However, with the continuous expansion of farming scale, disease outbreaks have become a major constraint to the development of the industry. Among the pathogens,\n                    <jats:italic>Edwardsiella tarda<\/jats:italic>\n                    is recognized as one of the primary causative agents. Identifying resistance\u2010related genes and applying marker\u2010assisted selection present promising strategies for breeding\n                    <jats:italic>E. tarda<\/jats:italic>\n                    \u2010resistant turbot strains. In this study, two families with the highest and lowest average breeding values were selected as resistant and susceptible groups, respectively. Transcriptomic analyses were conducted on spleen and head kidney tissues before and after\n                    <jats:italic>E. tarda<\/jats:italic>\n                    infection. The objective was to identify key genes associated with resistance to\n                    <jats:italic>E. tarda<\/jats:italic>\n                    in turbot. Among all comparison groups, the highest number of differentially expressed genes (DEGs) was observed in spleen tissue from sensitive families before and after infection (NiS vs. InS), with 866 significant DEGs. In contrast, the resistant group (NiR vs. InR) exhibited the fewest DEGs, with only 290 identified. Based on GO annotation and KEGG pathway enrichment, 26 significant DEGs were identified, including 7 upregulated genes (\n                    <jats:italic>gdf10a, tnfsf12, tat<\/jats:italic>\n                    ) and 19 downregulated genes (\n                    <jats:italic>jun, relb, fosab<\/jats:italic>\n                    ). In the KEGG pathway enrichment analysis, significant enrichment was observed in immune\u2010related pathways, including the MAPK signalling pathway, cytokine\u2010cytokine receptor interaction, toll\u2010like receptor signalling pathway, as well as metabolic pathways such as starch and sucrose metabolism, glycolysis\/gluconeogenesis and phenylalanine metabolism. Further analysis revealed that 21 of these genes were enriched in the MAPK signalling pathway and cytokine\u2013cytokine receptor interaction pathway, both of which are closely related to immune and inflammatory responses in fish. To verify the reliability of transcriptome data, four genes (\n                    <jats:italic>egfra, fosab, gdf10a<\/jats:italic>\n                    and\n                    <jats:italic>si_ch211\u201079\u2009m20.1<\/jats:italic>\n                    ) were selected for real\u2010time quantitative reverse transcription polymerase chain reaction (RT\u2010qPCR) validation. The results showed consistent expression trends with transcriptomic data, confirming the credibility of the identified DEGs. In conclusion, this study identified several candidate genes associated with resistance to\n                    <jats:italic>E. tarda<\/jats:italic>\n                    in turbot, enhancing our understanding of its immune response mechanisms and providing a theoretical basis for future genetic selection and molecular breeding efforts.\n                  <\/jats:p>","DOI":"10.1111\/jfb.70254","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T19:35:49Z","timestamp":1760556949000},"page":"528-542","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative transcriptome analysis reveals potential candidate genes for resistance to\n                    <i>Edwardsiella tarda<\/i>\n                    in turbot (\n                    <i>Scophthalmus maximus<\/i>\n                    )"],"prefix":"10.1111","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0896-5461","authenticated-orcid":false,"given":"Guanzheng","family":"Lyu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences  Qingdao China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology Center  Qingdao China"},{"name":"College of Fisheries and Life Sciences Shanghai Ocean University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ding","family":"Lyu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences  Qingdao China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology Center  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulong","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences  Qingdao China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology Center  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiji","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences  Qingdao China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology Center  Qingdao China"},{"name":"College of Fisheries and Life Sciences Shanghai Ocean University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2021.737643"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-10-r106"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19010245"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.10.2212"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(99)00309-1"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.48550\/arXiv.1505.02710"},{"issue":"4","key":"e_1_2_11_8_1","first-page":"382","article-title":"Isolation and identification of Edwardsiella tarda from diseased Japanese eel (Anguilla japonica)","volume":"17","author":"Chen C. 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