{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T02:06:18Z","timestamp":1779329178504,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T00:00:00Z","timestamp":1766361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T00:00:00Z","timestamp":1766361600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007403","name":"N\u00e1rodn\u00ed Agentura pro Zem\u011bd\u011blsk\u00fd V\u00fdzkum","doi-asserted-by":"publisher","award":["QK22010177"],"award-info":[{"award-number":["QK22010177"]}],"id":[{"id":"10.13039\/501100007403","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Non\u2010lethal diet sampling is essential where individual\u2010level monitoring and welfare matter, yet evidence on season\u2010long outcomes in agastric fishes is scarce. We tested syringe\u2010based gut flushing in pond\u2010reared common carp\n                    <jats:italic>Cyprinus carpio<\/jats:italic>\n                    over one growing season (April\u2013September) using PIT\u2010tagged fish assigned a priori to three handling histories: flushed (sedated, measured, flushed), handled\u2010only (sedated, measured), and unhandled controls. At harvest, recapture (survival proxy) remained high and statistically similar among handled groups and growth to harvest did not differ across treatments (end\u2010point mass: flushed 1857.3\u2009\u00b1\u2009367.4\u2009g; handled\u2010only 1769.7\u2009\u00b1\u2009311.8\u2009g; unhandled 1731.9\u2009\u00b1\u2009392.6\u2009g). A mid\u2010season divergence in July (handled\u2010only\u2009&gt;\u2009flushed in weight gain) was transient and absent at harvest. Operational performance scaled predictably: total water required to clear effluent increased with fish size (~20\u2009mL in small carp to &gt;90\u2009mL in large individuals; occasional ~120\u2009mL) and varied seasonally, rising from May to August and easing in September. Practical safeguards (rounded catheters guided between pharyngeal teeth; conservative advancement \u22642\u2009cm beyond the teeth; controlled bolus infusions; standardised recovery) supported complete evacuations with low injury risk. Collectively, these results validate gut flushing as a field\u2010ready, non\u2010lethal method that can be integrated into routine dietary monitoring of agastric cyprinids without compromising survival or harvest growth, while providing simple, size\u2010 and season\u2010based parameters to standardise protocols across studies.\n                  <\/jats:p>","DOI":"10.1111\/jfb.70301","type":"journal-article","created":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T07:48:10Z","timestamp":1766476090000},"page":"1192-1199","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Validating gut flushing as a non\u2010lethal sampling technique for diet monitoring in agastric fish"],"prefix":"10.1111","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2692-7811","authenticated-orcid":false,"given":"Lenka","family":"Kajgrova","sequence":"first","affiliation":[{"name":"Biology Centre CAS Institute of Hydrobiology  Ceske Budejovice Czech Republic"},{"name":"University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters  Vod\u0148any Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr","family":"Blabolil","sequence":"additional","affiliation":[{"name":"Biology Centre CAS Institute of Hydrobiology  Ceske Budejovice Czech Republic"},{"name":"University of South Bohemia in Ceske Budejovice Faculty of Science  Ceske Budejovice Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladislav","family":"Dra\u0161t\u00edk","sequence":"additional","affiliation":[{"name":"Biology Centre CAS Institute of Hydrobiology  Ceske Budejovice Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lubo\u0161","family":"Ko\u010dvara","sequence":"additional","affiliation":[{"name":"Biology Centre CAS Institute of Hydrobiology  Ceske Budejovice Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom\u00e1\u0161","family":"J\u016fza","sequence":"additional","affiliation":[{"name":"Biology Centre CAS Institute of Hydrobiology  Ceske Budejovice Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3597-6604","authenticated-orcid":false,"given":"Martin","family":"Bl\u00e1ha","sequence":"additional","affiliation":[{"name":"University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters  Vod\u0148any Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfb.14151"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v067.i01"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11160-012-9273-7"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fishres.2017.08.003"},{"key":"e_1_2_8_6_1","volume-title":"Quantifying diets of wildlife and fish: Practical and applied methods","author":"Calver M. 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Winter School on Towards Ecosystem Based Management of Marine Fisheries \u2013 Building Mass Balance Trophic and Simulation Models 1 148\u2013158."}],"container-title":["Journal of Fish Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfb.70301","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfb.70301","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfb.70301","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T01:14:53Z","timestamp":1779326093000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfb.70301"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,22]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1111\/jfb.70301"],"URL":"https:\/\/doi.org\/10.1111\/jfb.70301","archive":["Portico"],"relation":{},"ISSN":["0022-1112","1095-8649"],"issn-type":[{"value":"0022-1112","type":"print"},{"value":"1095-8649","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,22]]},"assertion":[{"value":"2025-05-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}