{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T10:04:38Z","timestamp":1784887478015,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The increasing use of fish in research emphasizes the need for effective and safe anaesthetic protocols, particularly for early life stages. Eugenol has been proposed as a promising anaesthetic, yet information regarding its effects on\n                    <jats:italic>Cnesterodon decemmaculatus<\/jats:italic>\n                    larvae remains scarce. This study assessed anaesthesia and recovery times, as well as gill tissue integrity, in larvae exposed to different eugenol concentrations. A randomized controlled trial was conducted using four treatment groups (6, 12.5, 25 and 50\u2009mgL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) and a control group (10\u2009mgL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ethanol). Significant differences were observed in both anaesthesia and recovery times across treatments (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Morphometric analyses showed that branchial filament area was significantly smaller in all eugenol\u2010treated groups compared to the control (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.02), and filament perimeter was also significantly reduced in all treatments (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Regarding necrosis, larvae exposed to 6 and 50\u2009mgL\n                    <jats:sup>\u22121<\/jats:sup>\n                    exhibited significantly more necrotic foci than controls (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Necrosis was highest at 6\u2009mgL\n                    <jats:sup>\u22121<\/jats:sup>\n                    , decreased at 12.5 and 25\u2009mgL\n                    <jats:sup>\u22121<\/jats:sup>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01) and increased again at 50\u2009mgL\n                    <jats:sup>\u22121<\/jats:sup>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). No significant differences were observed for oedema, congestion, lamellar fusion, epithelial lifting or vacuolization (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2265\u20090.08). However, oedema was higher at 6\u2009mgL\n                    <jats:sup>\u22121<\/jats:sup>\n                    than at 12.5\u2009mgL\n                    <jats:sup>\u22121<\/jats:sup>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.02), and congestion was lower at 12.5\u2009mgL\n                    <jats:sup>\u22121<\/jats:sup>\n                    than 25\u2009mgL\n                    <jats:sup>\u22121<\/jats:sup>\n                    (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.04). Overall, the results indicate that 12.5\u2009mgL\n                    <jats:sup>\u22121<\/jats:sup>\n                    eugenol provides effective anaesthesia with minimal histological alterations, representing the optimal concentration for use in\n                    <jats:italic>C. decemmaculatus<\/jats:italic>\n                    larvae.\n                  <\/jats:p>","DOI":"10.1111\/jfb.70378","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T10:30:12Z","timestamp":1772965812000},"page":"37-44","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Novel insights into eugenol anaesthesia in poeciliid larvae: Preliminary study of gill histology"],"prefix":"10.1111","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4927-3838","authenticated-orcid":false,"given":"Mar\u00eda C.","family":"Vercellini","sequence":"first","affiliation":[{"name":"Centro de Estudios Parasitol\u00f3gicos y de Vectores (CEPAVE) CONICET\u2010UNLP  La Plata Argentina"},{"name":"Facultad de Ciencias Veterinarias de la Universidad Nacional de la Plata (FCV\u2010UNLP)  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramiro","family":"Rearte","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias de la Universidad Nacional de la Plata (FCV\u2010UNLP)  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Carranza\u2010Mart\u00edn","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias de la Universidad Nacional de la Plata (FCV\u2010UNLP)  La Plata Argentina"},{"name":"Laboratorio de Reproducci\u00f3n Animal, Facultad de Ingenier\u00eda y Ciencias Agropecuarias (FICA) Pontificia Universidad Cat\u00f3lica (UCA)  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mat\u00edas H.","family":"Assandri","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Exactas de la Universidad Nacional de La Plata (FCE\u2010UNLP)  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvina","family":"Vargas","sequence":"additional","affiliation":[{"name":"Centro de Estudios Parasitol\u00f3gicos y de Vectores (CEPAVE) CONICET\u2010UNLP  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin M.","family":"Montes","sequence":"additional","affiliation":[{"name":"Centro de Estudios Parasitol\u00f3gicos y de Vectores (CEPAVE) CONICET\u2010UNLP  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel A.","family":"Ayala","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias de la Universidad Nacional de la Plata (FCV\u2010UNLP)  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0426.2010.01653.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jai.14361"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971-6580.75849"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/zeb.2019.1737"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enceco.2022.11.001"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.71.8.859"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.2000.tb02224.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/154585404774101671"},{"issue":"4","key":"e_1_2_9_10_1","first-page":"34","article-title":"Toxicity induced historical changes in gills of Oreochromis sp. by exposed to clove oil","volume":"9","author":"Hassan M.","year":"2015","journal-title":"Journal of Fisheries Sciences"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/raq.12245"},{"key":"e_1_2_9_12_1","first-page":"737","article-title":"Clove oil and menthol as anesthetic for platy","volume":"41","author":"Hoshiba M. 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