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Understanding how yellowfin tuna physiologically respond to environmental fluctuations is essential for revealing their adaptive strategies under stress conditions. In this study, two cold stress groups, LT (24\u00b0C) and ULT (18\u00b0C), were established, with a control group (CG, 30\u00b0C), and samples were collected at 0, 12, 24 and 36\u2009h. We systematically evaluated hepatic and serum responses of juvenile\n                    <jats:italic>T. albacares<\/jats:italic>\n                    to acute cold stress using liver transcriptome sequencing, histopathological analysis [haematoxylin and eosin (HE) staining], serum biochemical assays (cortisol, glucose, creatinine, phenoloxidase, total cholesterol, pyruvate kinase) and measurements of hepatic antioxidant [superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), glutathione peroxidase (GSH\u2010Px)] and metabolic enzyme [lactate dehydrogenase (LDH), alanine aminotransferase (ALT), alkaline phosphatase (AKP), acid phosphatase (ACP)] activities. Under 24\u00b0C stress, the fish primarily activated the \u2018cytokine\u2013cytokine receptor interaction\u2019 pathway, moderately adjusted SOD and GSH\u2010Px activities, showed minor fluctuations in cortisol, glucose and cholesterol levels and exhibited only mild vacuolation and oedema in liver tissue, suggesting a certain cold tolerance and maintenance of physiological homoeostasis. In contrast, 18\u00b0C stress resulted in significant enrichment of 16 KEGG pathways, including glycolysis, cholesterol metabolism, HIF\u20101 and PPAR signalling, indicating the mobilization of more complex stress responses. Enzyme activity analysis revealed suppressed antioxidant capacity, marked fluctuations in metabolic and transaminase activities and aggravated histological damage, such as hepatocyte oedema and necrosis. The findings reveal distinct adaptive mechanisms employed by yellowfin tuna when exposed to cold environments of differing severity.\n                  <\/jats:p>","DOI":"10.1111\/jfb.70455","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T05:52:12Z","timestamp":1777355532000},"page":"807-823","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of hepatic and serum responses to acute cold stress in juvenile yellowfin tuna\n                    <scp>\n                      <i>Thunnus albacares<\/i>\n                    <\/scp>\n                    (Bonnaterre 1788)"],"prefix":"10.1111","volume":"109","author":[{"given":"Junhua","family":"Huang","sequence":"first","affiliation":[{"name":"Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province Sanya Tropical Fisheries Research Institute  Sanya China"},{"name":"South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences  Guangzhou China"},{"name":"Hainan Fisheries Innovation Research Institute Chinese Academy of Fishery Sciences  Sanya China"},{"name":"Hainan Engineering Research Center for Deep\u2010sea Aquaculture and Processing Sanya Tropical Fisheries Research Institute  Sanya China"},{"name":"International Joint Research Center for Conservation and Application of Fishery Resources in the South China Sea Sanya Tropical Fisheries Research Institute  Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengyi","family":"Fu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province Sanya Tropical Fisheries Research Institute  Sanya China"},{"name":"South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences  Guangzhou China"},{"name":"Hainan Fisheries Innovation Research Institute Chinese Academy of 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