{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T02:16:42Z","timestamp":1787883402719,"version":"build-2784847793"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ft\u0131rma Kurumu","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The aim of this study was to investigate the potential effects of monosodium glutamate (MSG), a known flavour enhancer added to many ready\u2010to\u2010eat foods, as a feed additive in rainbow trout (\n                    <jats:italic>Oncorhynchus mykiss<\/jats:italic>\n                    ). Evaluating the effects of MSG on aquatic organisms will fill the knowledge gap in this field by monitoring the haematological indices of O. mykiss with growth parameters and somatic indices, histomap mapping with histopathological observations and determination of antioxidant\/cytokine enzyme levels via holistic\/multi\u2010biomarker approaches. Accordingly, rainbow trout feed was supplemented with MSG at different concentrations [(control (0% MSG), MSG\u2010I (0.25% MSG), MSG\u2010II (0.5% MSG), MSG\u2010III (0.75% MSG) and MSG\u2010IV (1% MSG)], and a 60\u2010day feeding trial was conducted. Considering the weight gain, MSG\u2010IV group showed an increase of 24.5% compared to the control and was determined as 61%. Exposure to different concentrations of MSG caused a decrease in erythrocyte, leukocyte, hemoglobin (Hg) and hematocrit (Hct) levels in\n                    <jats:italic>O. mykiss<\/jats:italic>\n                    . The effect of the same application on liver tissue, which is the detoxification organ, was determined as inhibition in antioxidant enzyme activities superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/www.google.com\/search?q=Nuclear+factor+erythroid+2-related+factor+2&amp;oq=NRF-2&amp;gs_lcrp=EgZjaHJvbWUqBwgAEAAYgAQyBwgAEAAYgAQyBwgBEAAYgAQyBwgCEAAYgAQyBwgDEAAYgAQyBwgEEAAYgAQyBwgFEAAYgAQyBwgGEAAYgAQyBwgHEAAYgAQyBggIEAAYHjIGCAkQABge0gEHNDM2ajBqN6gCALACAA&amp;sourceid=chrome&amp;ie=UTF-8&amp;ved=2ahUKEwik3YTiofmTAxVsUGwGHVegEGIQgK4QegYIAQgAEAM\">nuclear factor erythroid 2\u2010related factor 2<\/jats:ext-link>\n                    (NRF\u20102) level, induction in Reactive oxygen species (ROS) \u2010malondialdehyde (MDA) level, DNA damage, caspase\u20103, tumour necrosis factor \u03b1 (TNF\u2010\u03b1) and interleukin 6 (IL\u20106) activities. Histopathological examination of the liver and intestine revealed hydropic degeneration in a very small number of cells and mild hyperaemia of the blood vessels, with inflammation and DNA damage responses. With the reflection of this situation on histomap results, tissue damage profile and toxicity process differed based on dose and marker and manifested itself with mild\u2010to\u2010severe symptoms. These findings revealed that feeding with high concentrations of MSG was effective in NRF\u20102\/ROS pathways of oxidative stress and showed strong haemato\/hepatotoxic effects. Although all MSG concentrations applied were successful in terms of biomass measurements in terms of aquaculture, physiologically, the severity of toxic effects caused by MSG exposure was felt at a low level in liver tissues, and such pollutants should be considered in risk assessment.\n                  <\/jats:p>","DOI":"10.1111\/jfb.70460","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T02:20:44Z","timestamp":1777429244000},"page":"839-848","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Haematological and histopathological monitoring of monosodium glutamate\u2013supplemented feeding in rainbow trout (\n                    <i>Oncorhynchus mykiss<\/i>\n                    ): A possible modelling for a single health approach"],"prefix":"10.1111","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7539-1152","authenticated-orcid":false,"given":"Gonca","family":"Alak","sequence":"first","affiliation":[{"name":"Faculty of Fisheries Atat\u00fcrk University  Erzurum Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Furkan","family":"Bedir","sequence":"additional","affiliation":[{"name":"Faculty of Fisheries Atat\u00fcrk University  Erzurum Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asl\u0131 \u00c7ilingir","family":"Yeltekin","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry Van Yuzuncu Y\u0131l University  Van Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serkan","family":"Y\u0131ld\u0131r\u0131m","sequence":"additional","affiliation":[{"name":"Veterinary Faculty Ataturk University  Erzurum Turkey"},{"name":"Veterinary Faculty Kyrgyzstan\u2010T\u00fcrkiye Manas University  Bishkek 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