{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T02:16:52Z","timestamp":1787883412171,"version":"build-2784847793"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The twaite shad (\n                    <jats:italic>A. fallax<\/jats:italic>\n                    ), an anadromous fish species belonging to the Clupeidae family, has experienced population declines throughout Europe. This decline is attributable to a range of anthropogenic pressures, including migration barriers, environmental pollution, habitat degradation, predation by invasive species and unsustainable fishing practices. These factors disrupt the species' lifecycle and ecological balance, leading to significant conservation concerns. Typically, spawning occurs from late spring to early summer, which is when the migration might be up to 400\u2009km upstream from the sea. The demersal eggs hatch relatively quickly, and the larvae start a planktonic life in the river. Traditional monitoring methods have often proved inadequate due to the cryptic nature of\n                    <jats:italic>A. fallax<\/jats:italic>\n                    . With the goal of improving the efficacy of current biomonitoring designs, we tested an environmental DNA (eDNA) approach based on 12S rRNA gene Illumina sequencing to monitor\n                    <jats:italic>A. fallax<\/jats:italic>\n                    migration and spawning sites in the Po and Taro rivers in Northern Italy. This molecular approach was supplemented by scuba diving observations to visually confirm fish presence and habitat conditions at the same sites. Additionally, the collected larvae were morphologically identified and analysed with DNA barcoding to assess the correct taxonomic assignment of the species. This multi\u2010level framework confirmed the upstream migration and identified specific spawning sites, as well as the presence of larvae, highlighting the effectiveness of eDNA in detecting\n                    <jats:italic>A. fallax<\/jats:italic>\n                    populations. These findings underline the potential of eDNA as a powerful conservation tool for monitoring anadromous fish populations and guiding management strategies.\n                  <\/jats:p>","DOI":"10.1111\/jfb.70489","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T02:35:36Z","timestamp":1779762936000},"page":"1198-1209","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrating environmental\n                    <scp>DNA<\/scp>\n                    , diving surveys and\n                    <scp>DNA<\/scp>\n                    barcoding to track endangered twaite shad (\n                    <scp>\n                      <i>A<\/i>\n                    <\/scp>\n                    <i>losa fallax<\/i>\n                    ) spawning sites"],"prefix":"10.1111","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-0578-9738","authenticated-orcid":false,"given":"Alessia","family":"Ardenghi","sequence":"first","affiliation":[{"name":"Department of Chemistry, Life Sciences and Environmental Sustainability University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8047-959X","authenticated-orcid":false,"given":"Laura","family":"Filonzi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Life Sciences and Environmental Sustainability University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7451-5398","authenticated-orcid":false,"given":"Nicol\u00f2","family":"Bellin","sequence":"additional","affiliation":[{"name":"Department Biology University of Puerto Rico of Rio Piedras  San Juan Puerto Rico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro Maria","family":"Rontani","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Life Sciences and Environmental Sustainability University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0353-8389","authenticated-orcid":false,"given":"Matthew A.","family":"Campbell","sequence":"additional","affiliation":[{"name":"Trace and Environmental DNA (TrEnD) Laboratory. School of Molecular and Life Science Curtin University  Bentley Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6535-764X","authenticated-orcid":false,"given":"Mattia","family":"Sacc\u00f2","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Life Sciences and Environmental Sustainability University of Parma  Parma Italy"},{"name":"Trace and Environmental DNA (TrEnD) Laboratory. School of Molecular and Life Science Curtin University  Bentley Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morten E.","family":"Allentoft","sequence":"additional","affiliation":[{"name":"Trace and Environmental DNA (TrEnD) Laboratory. School of Molecular and Life Science Curtin University  Bentley Australia"},{"name":"Section for GeoGenetics Globe Institute, University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4731-9804","authenticated-orcid":false,"given":"Daniele","family":"Nizzoli","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Life Sciences and Environmental Sustainability University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armando","family":"Piccinini","sequence":"additional","affiliation":[{"name":"Gen\u2010Tech s.r.l.  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