{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T10:02:36Z","timestamp":1790244156157,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"Abstract<\/jats:title>\n \n Mitochondrial antiviral signalling protein (MAVS) is a critical adaptor in RIG\u2010I\u2010like receptor\u2010mediated antiviral innate immunity; however, its molecular characteristics and expression profiles in Chinese black sleeper (\n Bostrychus sinensis<\/jats:italic>\n ) remain poorly understood. In this study, the full\u2010length coding sequence of\n MAVS<\/jats:italic>\n from\n B. sinensis<\/jats:italic>\n (\n BsMAVS<\/jats:italic>\n ) was cloned and characterized. BsMAVS encodes 827 amino acid residues and contains a conserved caspase activation and recruitment domain (CARD). Multiple sequence alignment and phylogenetic analyses demonstrated that BsMAVS, particularly its CARD domain, is highly conserved among teleost species. Furthermore, qPCR analysis indicated that\n BsMAVS<\/jats:italic>\n is constitutively expressed in various tissues of healthy\n B. sinensis<\/jats:italic>\n , with relatively higher expression levels in immune\u2010related tissues, including the spleen and head kidney.\n BsMAVS<\/jats:italic>\n expression was dynamically regulated in the spleen, peripheral blood, liver and head kidney following polyinosinic\u2010polycytidylic acid (poly[I:C]) stimulation. These results provide baseline molecular and expression information for\n MAVS<\/jats:italic>\n in\n B. sinensis<\/jats:italic>\n , contributing to a better understanding of antiviral\u2010related genes in teleost fishes.\n <\/jats:p>","DOI":"10.1111\/jfb.70532","type":"journal-article","created":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T06:35:27Z","timestamp":1781332527000},"page":"1517-1527","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gene cloning, characterization and expression analysis of\n \n MAVS<\/i>\n <\/scp>\n in Chinese black sleeper (\n \n Bostrychus sinensis<\/i>\n <\/scp>\n )"],"prefix":"10.1111","volume":"109","author":[{"given":"Zhenzhen","family":"Zhou","sequence":"first","affiliation":[{"name":"College of Marine Resources and Environment Hebei Normal University of Science and Technology Qinhuangdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianshe","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center of Marine Facilities Aquaculture Zhejiang Ocean University Zhoushan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,12]]},"reference":[{"issue":"3","key":"e_1_2_9_2_1","first-page":"269","article-title":"The advancement of the biological characters and the artificial propagation of Bostrichtys sinesis","volume":"21","author":"Aihua Z.","year":"2002","journal-title":"Journal of Zhejiang Ocean University (Natural Science)"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.02.015"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2020.144361"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00404-09"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2014.10.017"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni921"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00293.2004"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2013.08.014"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2011.01.008"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R700002200"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2017.10.035"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04734"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2007.11.018"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.26392"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.12469"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25010181"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom15010139"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6807-8-11"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.08.012"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2016.06.032"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.04.056"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2010.08.012"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2010.11.010"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2023.07.008"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2019.11.067"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2021.106821"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2005.08.014"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2014.09.002"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2015.01.016"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2018.12.009"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2025.111077"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00251-003-0580-y"}],"container-title":["Journal of Fish Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfb.70532","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfb.70532","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfb.70532","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:05:25Z","timestamp":1790240725000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfb.70532"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,12]]},"references-count":32,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/jfb.70532"],"URL":"https:\/\/doi.org\/10.1111\/jfb.70532","archive":["Portico"],"relation":{},"ISSN":["0022-1112","1095-8649"],"issn-type":[{"value":"0022-1112","type":"print"},{"value":"1095-8649","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,12]]},"assertion":[{"value":"2026-01-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}