{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T04:41:22Z","timestamp":1792212082922,"version":"4.4.0"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The hypothalamic\u2013pituitary\u2010gonadal (HPG) axis, particularly follicle\u2010stimulating hormone (Fsh) and luteinizing hormone (Lh), is essential for the regulation of teleost reproduction. However, their expression dynamics and receptor distribution during gonadal development in silver pomfret remain poorly understood. This study aimed to characterize the expression profiles and cellular localization of Fsh\u03b2, Lh\u03b2 and their receptors (Fshr and Lhr) in the pituitary and gonads and to evaluate their potential roles in reproductive regulation. The different staining method and transmission electron microscopy (TEM) were used to analyse the structural changes of the pituitary gland during the reproductive season. Quantitative PCR (qPCR) and immunohistochemical (IHC) were used to detect the expression pattern of Fsh\u03b2 and Lh\u03b2. The results showed that the number of endocrine granules significantly increased before the reproductive season, and their electron density intensified. During the spawning season, the secretory granules gradually depleted, leading to the appearance of vacuolization. In the pituitary gland, the expression of\n                    <jats:italic>fsh\u03b2<\/jats:italic>\n                    in Stage IV was significantly higher in the female pituitary, but significantly higher in Stage V in the male. Fsh\u03b2 and Lh\u03b2 signals were mainly located in the proximal pars distalis (PPD) and pars intermedia (PI) regions of the pituitary. In the gonad, Fshr signals were observed in the spermatogonia type A cells and Sertoli cells in testes, whereas Lhr signals were observed in spermatogonia and secondary spermatocytes in the testes. Fshr and Lhr signals were both observed in the follicle cells surrounding the oogonia and vitellogenic oocytes in the ovary of silver pomfret. The expression patterns of\n                    <jats:italic>fsh\u03b2<\/jats:italic>\n                    and\n                    <jats:italic>fshr<\/jats:italic>\n                    were essentially similar after different hormone treatments in the gonad and methyltestosterone (MT) could influence the expression of\n                    <jats:italic>fsh\u03b2<\/jats:italic>\n                    ,\n                    <jats:italic>lh\u03b2<\/jats:italic>\n                    and\n                    <jats:italic>fshr<\/jats:italic>\n                    in the testis. Taken together, our data suggest that Fsh\u03b2 and Lh\u03b2 promote the development and maturation in the gonad, and Fshr and Lhr may be involved in ovary maturation and spawning.\n                  <\/jats:p>","DOI":"10.1111\/jfb.70576","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T13:30:08Z","timestamp":1786627808000},"page":"2357-2370","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The characteristics and expression profile of follicle\u2010stimulating hormone and luteinizing hormone during gonadal development in silver pomfret"],"prefix":"10.1111","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3425-2746","authenticated-orcid":false,"given":"Chunyang","family":"Guo","sequence":"first","affiliation":[{"name":"Country College of Marine Science Ningbo University  Ningbo China"},{"name":"Collaborative Innovation Center for Zhejiang Marine 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