{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T04:41:29Z","timestamp":1792212089353,"version":"4.4.0"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T00:00:00Z","timestamp":1786233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T00:00:00Z","timestamp":1786233600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004242","name":"Princess Nourah Bint Abdulrahman University","doi-asserted-by":"publisher","award":["PNURSP2026R84"],"award-info":[{"award-number":["PNURSP2026R84"]}],"id":[{"id":"10.13039\/501100004242","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study evaluated the protective role of dietary parsley seed powder (PSP) against acrylamide (ACR) toxicity in Nile tilapia (\n                    <jats:italic>Oreochromis niloticus<\/jats:italic>\n                    ). A total of 200 juvenile fish (15.98\u2009\u00b1\u20090.01\u2009g) were randomly assigned to four dietary groups (0.0, 2.5, 5.0 and 10.0\u2009g PSP\/kg diet) and exposed for 56\u2009days to a sublethal ACR concentration (3.46\u2009mg\/L). Throughout the experiment, growth performance, blood biochemical indices, immune responses, oxidative stress biomarkers, digestive enzyme activities, histopathology and hepatic gene expression were thoroughly assessed. The results revealed that PSP supplementation, particularly at 10.0\u2009g\/kg, significantly improved final body weight, body weight gained, daily weight gain and survival rate (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.05), while reducing feed conversion ratio significantly at 2.5\u2009g\/kg (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.001). Moreover, blood AST activity was significantly decreased in the 5.0 and 10.0\u2009g\/kg groups (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.01), accompanied by significantly elevated total protein and albumin (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01), and reduced uric acid and creatinine levels (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.001), indicating hepatoprotective and nephroprotective effects. Additionally, PSP exerted anti\u2010inflammatory and antioxidant benefits, as evidenced by significantly reduced serum tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) levels (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.001) and increased glutathione (GSH) concentration (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.05), especially in the 10.0\u2009g\/kg group. Digestive enzymes (amylase and pepsin) exhibited a significant increase at all PSP levels (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.05 or 0.01), with the highest levels observed in the DP\n                    <jats:sub>3<\/jats:sub>\n                    group (10.0\u2009g PSP\/kg diet). Histopathological evaluation indicated a dose\u2010dependent attenuation of intestinal and hepatopancreatic alterations with increasing PSP levels, as evidenced by reduced lesion severity, decreased necrotic changes and better preservation of tissue architecture. Furthermore, gene expression analysis revealed significant downregulation of cysteine\u2013aspartic acid protease\u20103 (\n                    <jats:italic>caspase\u20103<\/jats:italic>\n                    ) and tumour necrosis factor\u2010alpha 2 (\n                    <jats:italic>tnf\u20102\u03b1<\/jats:italic>\n                    ) genes (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.01) and upregulation of insulin\u2010like growth factor 1 (\n                    <jats:italic>igf\u20101<\/jats:italic>\n                    ) and growth hormone (\n                    <jats:italic>gh<\/jats:italic>\n                    ) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.01) genes in the 10.0\u2009g\/kg group, indicating enhanced growth and cellular resilience. In conclusion, dietary PSP at 10.0\u2009g\/kg significantly alleviates ACR toxicity by enhancing physiological, biochemical, immunological and molecular responses in Nile tilapia.\n                  <\/jats:p>","DOI":"10.1111\/jfb.70606","type":"journal-article","created":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T23:41:41Z","timestamp":1786318901000},"page":"2294-2310","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary parsley seed powder attenuates acrylamide\u2010waterborne toxicity hazards on growth, redox status, immunity and gene regulations in\n                    <i>Oreochromis niloticus<\/i>"],"prefix":"10.1111","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8172-5366","authenticated-orcid":false,"given":"Mohammed A. E.","family":"Naiel","sequence":"first","affiliation":[{"name":"Department of Animal Production, Faculty of Agriculture Zagazig University  Zagazig Sharqia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman M.","family":"Zaki","sequence":"additional","affiliation":[{"name":"Reproduction and Fish Physiology Department Central Laboratory for Aquaculture Research, Agricultural Research Centre  Abbassa, Abo\u2010Hammad Sharqia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2922-1053","authenticated-orcid":false,"given":"Taghrid M. N.","family":"Abdelhakim","sequence":"additional","affiliation":[{"name":"Department of Fish Health and Management Central Laboratory for Aquaculture Research, Agricultural Research Centre  Abbassa, Abo\u2010Hammad Sharqia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud","family":"Moustafa","sequence":"additional","affiliation":[{"name":"Department of Biology College of Science, Princess Nourah bint Abdulrahman University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Al\u2010Shehri","sequence":"additional","affiliation":[{"name":"Department of Biology College of Science, Princess Nourah bint Abdulrahman University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayasar I.","family":"Al\u2010zaban","sequence":"additional","affiliation":[{"name":"Department of Biology College of Science, Princess Nourah bint Abdulrahman University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahla. 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