{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T04:41:25Z","timestamp":1792212085330,"version":"4.4.0"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100014734","name":"Japan Fisheries Research and Education Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014734","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004620","name":"Fisheries Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004620","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Selective breeding of yellowtail (\n                    <jats:italic>Seriola quinqueradiata<\/jats:italic>\n                    ) is expanding in Japan, increasing the demand for practical reproductive control tools in commercial aquaculture to reduce genetic risks from escapees. Slowing early gonadal development through environmental manipulation may support future sterilization strategies, particularly when combined with other techniques. This study aimed to examine somatic growth and early gonadal development in juvenile yellowtail reared under continuous darkness (0L:24D). Juveniles were reared under 0L:24D or a 12L:12D photoperiod (control) and sampled at Weeks 0, 2, 4, 6, 8 and 10. Growth was recorded at each time point, and gonads were examined by histology and immunohistochemistry to assess gonadal sex differentiation, germ\u2010cell proliferation and onset of meiosis. Water temperature increased similarly in both groups and exceeded 28\u00b0C from Week 6 onward. Despite identical feeding rations, fish reared under 0L:24D were significantly larger at Weeks 8 and 10 than controls. Gonadal sex differentiation progressed normally in both groups, and germ\u2010cell proliferative activity did not differ between groups. SYCP3\u2010positive germ cells were not detected at Week 6 under 0L:24D, whereas the proportion of SYCP3\u2010positive germ cells at Weeks 8 to 10 was comparable to that in controls, suggesting a possible transient difference in the initial appearance of SYCP3\u2010positive germ cells. In females, fish reared under 0L:24D showed lower area fraction and diameter of chromatin nucleolus\u2010 and perinucleolus\u2010stage (CN\/PN) oocytes at Weeks 8 and 10, suggesting reduced development of CN\/PN\u2010stage oocytes. These results suggest that, under the present single\u2010tank experimental conditions, rearing under continuous darkness was associated with improved growth performance during the high\u2010temperature period and sex\u2010specific changes in early gonadal development in juvenile yellowtail.\n                  <\/jats:p>","DOI":"10.1111\/jfb.70617","type":"journal-article","created":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T02:55:41Z","timestamp":1786762541000},"page":"2371-2382","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Continuous darkness is associated with improved growth performance during a high\u2010temperature period and sex\u2010specific modulation of early gonadal development in juvenile yellowtail (\n                    <i>Seriola quinqueradiata<\/i>\n                    )"],"prefix":"10.1111","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-4081-4054","authenticated-orcid":false,"given":"Yuya","family":"Hasegawa","sequence":"first","affiliation":[{"name":"Fisheries Technology Institute, Japan Fisheries Research and Education Agency (FRA)  Nagasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiko","family":"Shibata","sequence":"additional","affiliation":[{"name":"Fisheries Technology Institute, Japan Fisheries Research and Education Agency (FRA)  Nagasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiga","family":"Abe","sequence":"additional","affiliation":[{"name":"Fisheries Technology Institute, Japan Fisheries Research and Education Agency (FRA)  Nagasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takurou","family":"Hotta","sequence":"additional","affiliation":[{"name":"Fisheries Technology Institute, Japan Fisheries Research and Education Agency 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