{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T01:45:15Z","timestamp":1791769515286,"version":"4.3.4"},"reference-count":41,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Acrossocheilus fasciatus<\/jats:italic>\n                    is an economically important freshwater fish species in China. In recent years,\n                    <jats:italic>Citrobacter freundii<\/jats:italic>\n                    infection has caused significant harm to the aquaculture industry. In this study, we performed histological observation and RNA\u2010seq analysis of the intestines and spleens of control (CK) and\n                    <jats:italic>C. freundii<\/jats:italic>\n                    infected\n                    <jats:italic>A. fasciatus<\/jats:italic>\n                    (EG). The results showed that infected fish exhibited abdominal haemorrhage, intestinal villous shedding, hydropic degeneration of hepatocytes and splenic sinus dilation with congestion. RNA\u2010seq analysis identified 3054 DEGs in the intestine and 7706 DEGs in the spleen, with 1247 overlapping DEGs identified by combined analysis. A substantial portion of these genes clustered within biological activities linked to immunity and metabolic regulation, particularly engaging immune\u2010related routes such as endoplasmic reticulum\u2010based protein maturation, alongside the complement system and coagulation signalling cascades. This study systematically reveals the synergy and division of labor between the intestine and spleen during\n                    <jats:italic>C. freundii<\/jats:italic>\n                    infection, providing fundamental data for understanding the immune response mechanisms of\n                    <jats:italic>A. fasciatus<\/jats:italic>\n                    and a theoretical basis for optimising disease prevention and control strategies.\n                  <\/jats:p>","DOI":"10.1111\/jfb.70663","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T01:16:57Z","timestamp":1791767817000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomic analysis reveals the immune response mechanisms of\n                    <i>Acrossocheilus fasciatus<\/i>\n                    against\n                    <i>Citrobacter freundii<\/i>\n                    infection"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7050-4250","authenticated-orcid":false,"given":"Zihang","family":"Wu","sequence":"first","affiliation":[{"name":"College of Life Sciences Zhejiang Normal University  Jinhua China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2320-230X","authenticated-orcid":false,"given":"Wenbin","family":"Fang","sequence":"additional","affiliation":[{"name":"College of Life Sciences Zhejiang Normal University  Jinhua China"},{"name":"Key Lab of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province Zhejiang Normal University  Jinhua China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6738-8332","authenticated-orcid":false,"given":"Renxuan","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Life Sciences Zhejiang Normal University  Jinhua China"},{"name":"Key Lab of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province Zhejiang Normal University  Jinhua China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2574-3164","authenticated-orcid":false,"given":"Shanjian","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Life Sciences Zhejiang Normal University  Jinhua China"},{"name":"Key Lab of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province Zhejiang Normal University  Jinhua China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,11]]},"reference":[{"issue":"2","key":"e_1_2_11_2_1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.jaci.2025.03.024","article-title":"Inside job: Roles of intracellular C3","volume":"156","author":"Afzali B.","year":"2025","journal-title":"Journal of Allergy and Clinical Immunology"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.micpath.2018.09.038","article-title":"Citrobacter freundii infection in silver catfish (Rhamdia quelon): Hematological and histological alterations","volume":"125","author":"Bandeira Junior G.","year":"2018","journal-title":"Microbial Pathogenesis"},{"issue":"1","key":"e_1_2_11_4_1","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1158\/2326-6066.CIR-24-0320","article-title":"Alternate MHC I antigen presentation pathways allow Cd8+ T\u2010cell recognition and killing of cancer cells in the absence of \u03b22M or TAP","volume":"13","author":"Cruz F. M.","year":"2025","journal-title":"Cancer Immunology Research"},{"issue":"6","key":"e_1_2_11_5_1","doi-asserted-by":"crossref","DOI":"10.1007\/s10499-025-02222-2","article-title":"Citrobacter freundii induced pathogenicity towards fish: An insight investigation through serum biochemistry and gene expression analysis in Labeo rohita","volume":"33","author":"Dhar S.","year":"2025","journal-title":"Aquaculture International"},{"issue":"2","key":"e_1_2_11_6_1","doi-asserted-by":"crossref","DOI":"10.48045\/001c.122390","article-title":"First report of Citrobacter freundii infection in exotic Tropheus spp","volume":"44","author":"Dincturk E.","year":"2024","journal-title":"Bulletin of the European Association of Fish Pathologists"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.aqrep.2025.102724","article-title":"Mechanisms of immune responses in juvenile Acanthopagrus latus to Aeromonas hydrophila infection revealed by multi\u2010tissue transcriptomic analysis","volume":"41","author":"Fan H. D.","year":"2025","journal-title":"Aquaculture Reports"},{"key":"e_1_2_11_8_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2025.110114","article-title":"Mechanisms of immune responses in Acanthopagrus latus to Streptococcus agalactiae infection revealed by transcriptomic analysis","volume":"157","author":"Fan H. D.","year":"2025","journal-title":"Fish & Shellfish Immunology"},{"key":"e_1_2_11_9_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2025.147758","article-title":"Comparative evaluation of nutritional quality and flavor characteristics for Acrossocheilus fasciatus muscle in different aquaculture systems","volume":"502","author":"Fan H. J.","year":"2026","journal-title":"Food Chemistry"},{"issue":"6","key":"e_1_2_11_10_1","first-page":"44","article-title":"Isolation, identification and drug resistance analysis of Citrobacter freundii from Acrossocheilus fasciatus","volume":"38","author":"Fang W. B.","year":"2025","journal-title":"Chinese Journal of Fisheries"},{"key":"e_1_2_11_11_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2025.111061","article-title":"Integrated analysis of Dna methylome and transcriptome reveals key regulatory genes and immune pathways in Opsariichthys bidens against Aeromonas veronii infection","volume":"169","author":"Fang W. B.","year":"2025","journal-title":"Fish & Shellfish Immunology"},{"key":"e_1_2_11_12_1","first-page":"183738","article-title":"Induction of apoptosis coupled to endoplasmic reticulum stress through regulation of chop and Jnk in bone marrow mesenchymal stem cells from patients with systemic lupus erythematosus","volume":"2015","author":"Guo G. 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