{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:59:15Z","timestamp":1790762355754,"version":"4.1.0"},"reference-count":67,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Intragastric gavage is widely used in studies of digestive physiology to deliver standardised meals, yet it remains unclear whether it elicits postprandial metabolic and acid\u2013base responses comparable to voluntary feeding. Here, we tested whether the method of meal delivery affects the magnitude and timing of the postprandial rise in metabolic rate known as specific dynamic action (SDA) and the associated alkaline tide (transient rise in blood pH and bicarbonate following feeding) in sand flathead (\n                    <jats:italic>Platycephalus bassensis<\/jats:italic>\n                    ) using a standardised 1.5% body\u2010weight ratio either as intact pellets (voluntary feeding) or as a homogenised slurry via gavage, alongside fasted and sham\u2010gavage controls. SDA magnitude was ~3.6\u2010fold greater and duration was ~37\u2009h longer in voluntarily fed fish. Removing the post\u2010handling period reduced this difference, and the early post\u2010entry period accounted for a greater proportion of metabolic expenditure in voluntarily fed fish, suggesting apparent differences partly reflect procedural effects of feeding method, including the mandated use of anaesthesia during the gavage procedure. Voluntarily fed fish showed a clear alkaline tide, to our knowledge the first report in this species, whereas gavage\u2010fed fish showed no tide at 6\u2009h, with lower blood pH and a delayed bicarbonate rise. Gut content distribution at 6, 12 and 24\u2009h post feeding indicated reduced temporal separation between gastric acid secretion and intestinal neutralisation in gavage\u2010fed fish. These results show that method choice can modify digestive physiology in ways that should be considered when interpreting and comparing studies of postprandial responses in fish.\n                  <\/jats:p>","DOI":"10.1111\/jfb.70678","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:02:42Z","timestamp":1790758962000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Method of meal delivery influences estimates of postprandial metabolism and alkaline tide in a benthic fish"],"prefix":"10.1111","author":[{"given":"Junlin","family":"Chen","sequence":"first","affiliation":[{"name":"Institute for Marine and Antarctic Studies (IMAS), University of Tasmania  Hobart Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9406-0578","authenticated-orcid":false,"given":"Barrett W.","family":"Wolfe","sequence":"additional","affiliation":[{"name":"Institute for Marine and Antarctic Studies (IMAS), University of Tasmania  Hobart Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2568-0562","authenticated-orcid":false,"given":"Harriet R.","family":"Goodrich","sequence":"additional","affiliation":[{"name":"Institute for Marine and Antarctic Studies (IMAS), University of Tasmania  Hobart Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00226505"},{"issue":"11","key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"1877","DOI":"10.1093\/clinchem\/29.11.1877","article-title":"Temperature correction of blood\u2010gas and pH measurements","volume":"29","author":"Ashwood E. 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