{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T01:45:20Z","timestamp":1791769520817,"version":"4.3.4"},"reference-count":17,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Fish eye lenses offer a chronological archive of individual life histories and preserve genomic DNA. However, integrating ecological and genetic data is hindered by post\u2010mortem nuclease degradation, and standardized DNA preservation protocols for this tissue remain undefined. We evaluated chemical and thermal preservation methods to maximize DNA yield and integrity from fish lenses. Using a paired experimental design across three marine species, we demonstrate that chemical stabilization with TRIzol yields significantly higher DNA quantities (2654.7\u2009\u00b1\u2009331.5\u2009ng) than ultra\u2010low temperature freezing at \u221280\u00b0C (1425.4\u2009\u00b1\u2009866.6\u2009ng) or standard freezing at \u221220\u00b0C. TRIzol denatures endogenous nucleases upon contact, preventing the enzymatic fragmentation associated with passive freezing. This optimized protocol establishes a methodological standard for eco\u2010isotopic genomics, enabling the direct coupling of phenotypic chronologies with genotypes to assess marine population resilience.<\/jats:p>","DOI":"10.1111\/jfb.70679","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T01:19:11Z","timestamp":1791767951000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Active nuclease inhibition over passive freezing secures the genomic archive of fish eye lenses"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9895-1469","authenticated-orcid":false,"given":"Sebastian A.","family":"Klarian","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n Marina Quintay CIMARQ. Facultad de Ciencias de la Vida Universidad Andres Bello  Vi\u00f1a del Mar Chile"},{"name":"Department of Ecology and Evolutionary Biology, U\u20103043 University of Connecticut  Storrs Connecticut USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5368-4475","authenticated-orcid":false,"given":"Ruben","family":"Avenda\u00f1o\u2010Herrera","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Marina Quintay CIMARQ. Facultad de Ciencias de la Vida Universidad Andres Bello  Vi\u00f1a del Mar Chile"},{"name":"Laboratorio de Organismos Acu\u00e1ticos y Biotecnolog\u00eda Acu\u00edcola Universidad Andres Bello  Vi\u00f1a del Mar Chile"},{"name":"Centro FONDAP INCAR Universidad Andres Bello  Vi\u00f1a del Mar Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0615-820X","authenticated-orcid":false,"given":"Juan Antonio","family":"Valdes","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Marina Quintay CIMARQ. Facultad de Ciencias de la Vida Universidad Andres Bello  Vi\u00f1a del Mar Chile"},{"name":"Centro FONDAP INCAR Universidad Andres Bello  Vi\u00f1a del Mar Chile"},{"name":"Departamento de Ciencias Biol\u00f3gicas. Facultad de Ciencias de la Vida Universidad Andres Bello  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4256-9559","authenticated-orcid":false,"given":"Juan","family":"Vargas\u2010Perez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Marina Quintay CIMARQ. Facultad de Ciencias de la Vida Universidad Andres Bello  Vi\u00f1a del Mar Chile"},{"name":"Programa de Doctorado en Medicina de la Conservaci\u00f3n. Facultad de Ciencias de la Vida Universidad Andres Bello  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6358-255X","authenticated-orcid":false,"given":"Carolina","family":"C\u00e1rcamo","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Marina Quintay CIMARQ. Facultad de Ciencias de la Vida Universidad Andres Bello  Vi\u00f1a del Mar Chile"},{"name":"Universidad de Antofagasta Stable Isotope Facility (UASIF), Instituto Antofagasta de Recursos Naturales Renovables Universidad de Antofagasta  Antofagasta Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9664-3132","authenticated-orcid":false,"given":"Henry","family":"Araya\u2010Le\u00f3n","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Marina Quintay CIMARQ. Facultad de Ciencias de la Vida Universidad Andres Bello  Vi\u00f1a del Mar Chile"},{"name":"Laboratorio de Organismos Acu\u00e1ticos y Biotecnolog\u00eda Acu\u00edcola Universidad Andres Bello  Vi\u00f1a del Mar Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2252-7746","authenticated-orcid":false,"given":"Francisco","family":"Fernandoy","sequence":"additional","affiliation":[{"name":"Laboratorio de An\u00e1lisis Isot\u00f3pico. Facultad de Ingenier\u00eda Universidad Andres Bello  Vi\u00f1a del Mar Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,11]]},"reference":[{"key":"e_1_2_9_2_1","article-title":"Development of methods for obtaining high\u2010quality DNA from ocular tissues","volume":"65","author":"Ashby C. K.","year":"2024","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/s41576-019-0157-8","article-title":"DNA as a biological lens","volume":"20","author":"Burgess D. J.","year":"2019","journal-title":"Nature Reviews. Genetics"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1111\/jfb.70413","article-title":"Lessons learned from applying eDNA surveying to diadromous fish detection across the Northeast Atlantic region","volume":"109","author":"Claver C.","year":"2026","journal-title":"Journal of Fish Biology"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"181","DOI":"10.3354\/meps13247","article-title":"Stable isotope analysis of eye lenses from invasive lionfish yields record of resource use","volume":"637","author":"Curtis J. S.","year":"2020","journal-title":"Marine Ecology Progress Series"},{"key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"183","DOI":"10.3354\/meps13671","article-title":"Life history through the eyes of a hogfish: Trophic growth and differential juvenile habitat use from stable isotope analysis","volume":"666","author":"Faletti M. E.","year":"2021","journal-title":"Marine Ecology Progress Series"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1111\/jfb.14248","article-title":"Rapid cooling via dry ice preserves the genetic and morphological integrity of fish embryos","volume":"96","author":"Fost B.","year":"2020","journal-title":"Journal of Fish Biology"},{"key":"e_1_2_9_8_1","unstructured":"Invitrogen. (2016).TRIzol\u2122 Reagent. Experimental protocol for DNA isolation. Thermo Fisher Scientific."},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecss.2019.106248","article-title":"Atlantic tarpon (Megalops atlanticus) exhibit upper estuarine habitat dependence followed by foraging system fidelity after ontogenetic habitat shifts","volume":"225","author":"Kurth B. N.","year":"2019","journal-title":"Estuarine, Coastal and Shelf Science"},{"key":"e_1_2_9_10_1","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1007\/s12686-013-9893-9","article-title":"Evaluation of three methods for high throughput extraction of DNA from challenging fish tissues","volume":"5","author":"Meissner H.","year":"2013","journal-title":"Conservation Genetics Resources"},{"key":"e_1_2_9_11_1","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1021\/acs.estlett.2c00755","article-title":"Tracking fish lifetime exposure to mercury using eye lenses","volume":"10","author":"Miraly H.","year":"2022","journal-title":"Environmental Science & Technology Letters"},{"key":"e_1_2_9_12_1","doi-asserted-by":"crossref","first-page":"8643","DOI":"10.1002\/ece3.6558","article-title":"DNA degradation in fish: Practical solutions and guidelines to improve DNA preservation for genomic research","volume":"10","author":"Oosting T.","year":"2020","journal-title":"Ecology and Evolution"},{"key":"e_1_2_9_13_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/15476286.2025.2483118","article-title":"Nucleolar ribosomal RNA synthesis continues in differentiating lens fiber cells until abrupt nuclear degradation required for ocular lens transparency","volume":"22","author":"Ray\u00eae D.","year":"2025","journal-title":"RNA Biology"},{"key":"e_1_2_9_14_1","doi-asserted-by":"crossref","DOI":"10.3390\/ani10091585","article-title":"From land to water: Taking fish welfare seriously","volume":"10","author":"S\u00e1nchez\u2010Su\u00e1rez W.","year":"2020","journal-title":"Animals: An Open Access Journal from MDPI"},{"key":"e_1_2_9_15_1","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1002\/lom3.10153","article-title":"Chemical archives in fishes beyond otoliths: A review on the use of other body parts as chronological recorders of microchemical constituents for expanding interpretations of environmental, ecological, and life\u2010history changes","volume":"15","author":"Tzadik O. E.","year":"2017","journal-title":"Limnology and Oceanography, Methods\/ASLO"},{"key":"e_1_2_9_16_1","doi-asserted-by":"crossref","first-page":"107047","DOI":"10.1016\/j.ecss.2020.107047","article-title":"Spawning origins and ontogenetic movements for demersal fishes: An approach using eye\u2010lens stable isotopes","volume":"246","author":"Vecchio J. L.","year":"2020","journal-title":"Estuarine, Coastal and Shelf Science"},{"key":"e_1_2_9_17_1","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1038\/s41592-019-0686-2","article-title":"SciPy 1.0: Fundamental algorithms for scientific computing in Python","volume":"17","author":"Virtanen P.","year":"2020","journal-title":"Nature Methods"},{"key":"e_1_2_9_18_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0108935","article-title":"Stable isotopes in fish eye lenses as potential recorders of trophic and geographic history","volume":"9","author":"Wallace A. A.","year":"2014","journal-title":"PLoS One"}],"container-title":["Journal of Fish Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfb.70679","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfb.70679","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfb.70679","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T01:19:19Z","timestamp":1791767959000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfb.70679"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,11]]},"references-count":17,"alternative-id":["10.1111\/jfb.70679"],"URL":"https:\/\/doi.org\/10.1111\/jfb.70679","archive":["Portico"],"relation":{},"ISSN":["0022-1112","1095-8649"],"issn-type":[{"value":"0022-1112","type":"print"},{"value":"1095-8649","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,11]]},"assertion":[{"value":"2026-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"jfb.70679"}}