{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T23:39:48Z","timestamp":1792366788074,"version":"4.4.0"},"reference-count":86,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:00:00Z","timestamp":1792281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:00:00Z","timestamp":1792281600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Biology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Largemouth bass (\n                    <jats:italic>Micropterus salmoides<\/jats:italic>\n                    ) is a globally significant freshwater aquaculture species known for its rapid growth, high\u2010quality flesh and excellent nutritional value. Given the increasing incidence of hypoxia in its aquaculture setting, understanding the molecular mechanisms underlying gill adaptation to hypoxic stress is critical for improving resilience in this species. To achieve this, we employed full\u2010length transcript sequencing using PacBio Iso\u2010Seq and high\u2010throughput Illumina RNA\u2010Seq to construct a comprehensive transcriptome profile. Histological analyses revealed apparent structural alterations in gill tissues under hypoxic stress (dissolved oxygen: 2.0\u2009\u00b1\u20090.5\u2009mg\/L, duration: 96\u2009h), including curved and swollen gill filaments, apoptosis and epithelial cell vacuolation. Comparative transcriptomic analysis revealed pronounced shifts in the expression of genes involved in energy metabolism, angiogenesis, immune responses, cell proliferation and apoptosis. Notably, the down\u2010regulation of genes associated with cell cycle and proliferation suggests that\n                    <jats:italic>M. salmoides<\/jats:italic>\n                    may conserve energy by suppressing cell division under hypoxic conditions. In contrast, the increased expression of apoptosis\u2010related genes, including\n                    <jats:italic>casp7<\/jats:italic>\n                    , indicates that apoptosis may play a protective role in maintain tissue homeostasis under hypoxia. Furthermore, the suppression of chemokine\u2010mediated immune pathways implies that hypoxia redirects energy toward essential metabolic processes at the cost of local immune responses. GO and KEGG enrichment analyses identified TNF\u2010\u03b1 and chemokine\u2010mediated signalling pathways as key pathways involved in tissue homeostasis and adaptive responses to hypoxic stress. These findings enhance our understanding of the mechanisms underlying hypoxia adaptation and provide valuable genetic resources for breeding strategies aimed at enhancing hypoxia tolerance in\n                    <jats:italic>M. salmoides<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/jfb.70697","type":"journal-article","created":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T23:01:06Z","timestamp":1792364466000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated transcriptomics and histological analysis reveal gill adaptation mechanisms to hypoxic stress in largemouth bass (\n                    <i>Micropterus salmoides<\/i>\n                    )"],"prefix":"10.1111","author":[{"given":"Xiaoli","family":"Ma","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences Jiangsu Normal University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9633-2733","authenticated-orcid":false,"given":"Baofeng","family":"Su","sequence":"additional","affiliation":[{"name":"School of Fisheries, Aquaculture and Aquatic Sciences Auburn University  Auburn Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anxin","family":"Ye","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences Jiangsu Normal University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9892-8017","authenticated-orcid":false,"given":"Renyu","family":"Wan","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences Jiangsu Normal University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences Jiangsu Normal University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4452-8424","authenticated-orcid":false,"given":"Liang","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences City University of Hong Kong  Kowloon Tong Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxin","family":"Yu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences Jiangsu Normal University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhe","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences Jiangsu Normal University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyi","family":"Wu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences Jiangsu Normal University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0562-6363","authenticated-orcid":false,"given":"Wenlong","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences City University of Hong Kong  Kowloon Tong Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1007\/s10695-019-00614-9","article-title":"Fish response to hypoxia stress: Growth, physiological, and immunological biomarkers","volume":"45","author":"Abdel\u2010Tawwab M.","year":"2019","journal-title":"Fish Physiology and Biochemistry"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","DOI":"10.1534\/g3.120.401487","volume-title":"Identification of hypoxia\u2010specific biomarkers in salmonids using RNA\u2010sequencing and validation using high\u2010throughput qPCR","author":"Akbarzadeh A.","year":"2020"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1261\/rna.051557.115","article-title":"Leveraging transcript quantification for fast computation of alternative splicing profiles","volume":"21","author":"Alamancos G. 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