{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T23:00:51Z","timestamp":1784242851814,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,10,24]],"date-time":"2012-10-24T00:00:00Z","timestamp":1351036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Marine cultured meagre, <jats:italic>Argyrosomus regius<\/jats:italic> Asso, in central and western Greece were affected by an outbreak of systemic granulomatous disease subsequently demonstrated to be nocardiosis. The fish were originally imported as juveniles from hatcheries in France and Italy and on\u2010grown in Greece, the latter also providing broodstock for a small number of local Greek hatcheries for the production of second\u2010generation juveniles. The disease in cage reared fish had been present throughout the year, particularly in the 1+ and 2+ year old fish with a low to variable morbidity and 1\u20134% total mortality. Multiple lesions were visible externally on the skin of affected fish, with severe ulcerations and necrosis. Internally, multifocal yellowish\u2010white nodules, 0.1\u20130.5\u00a0cm in diameter, were visible on the surface of several internal organs. Histopathology revealed systemic granulomatous inflammation. Fite\u2010Faraco staining clearly demonstrated the presence of <jats:italic>Nocardia<\/jats:italic>\u2010like organisms which were Gram\u2010positive, long, rod to beaded filamentous bacteria. <jats:italic>Nocardia<\/jats:italic> genus\u2010specific 16s <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> primers <jats:styled-content style=\"fixed-case\">NG<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">NG<\/jats:styled-content>2 were used to generate a 600\u00a0bp fragment recovered from affected tissue, confirming the diagnosis of <jats:italic>Nocardia<\/jats:italic> spp. To our knowledge, this is the first report of nocardiosis in meagre.<\/jats:p>","DOI":"10.1111\/jfd.12015","type":"journal-article","created":{"date-parts":[[2012,10,24]],"date-time":"2012-10-24T10:31:06Z","timestamp":1351074666000},"page":"141-149","source":"Crossref","is-referenced-by-count":44,"title":["Systemic nocardiosis in a Mediterranean population of cultured meagre, <i>Argyrosomus regius<\/i> Asso (Perciformes: Sciaenidae)"],"prefix":"10.1111","volume":"36","author":[{"given":"A","family":"Elkesh","sequence":"first","affiliation":[{"name":"Institute of Aquaculture University of Stirling Stirling UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K P L","family":"Kantham","sequence":"additional","affiliation":[{"name":"Nireus S A Hiliadou Doridos Focida Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A P","family":"Shinn","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture University of Stirling Stirling UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Crumlish","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture University of Stirling Stirling UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R H","family":"Richards","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture University of Stirling Stirling UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,10,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2761.2003.00485.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(96)01475-5"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2009.10.002"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1128\/jb.170.3.1137-1142.1988","article-title":"Effect of growth stage on mycolic acid structure in cell walls of Nocardia asteroides GUH\u20102","volume":"170","author":"Beaman B.L.","year":"1988","journal-title":"Journal of Bacteriology"},{"key":"e_1_2_6_6_1","first-page":"22","article-title":"Diseases of Mediterranean fish species: Problems, research and prospects","volume":"24","author":"Colorni A.","year":"2004","journal-title":"Bulletin of the European Association of Fish Pathologists"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2011.01269.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2007.00779.x"},{"key":"e_1_2_6_9_1","unstructured":"DegolladaE. DomingoM. AlonsoJ.M. AlegreF. TelloM.&LopezA.(1996)Nocardiosis in a striped dolphin (Stenella coeruleoalba). 10th Annual Conference of the European Cetacean Society Lisboa. Abstract."},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"2142","DOI":"10.1128\/jcm.28.9.2142-2144.1990","article-title":"Value of the O\u2010nitrophenyl\u2010beta\u2010D galactopyranoside test to differentiate among the aerobic actinomycetes","volume":"28","author":"Flores M.","year":"1990","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_6_11_1","unstructured":"KanthamK.P.L.&SboukisA.(2010)Natural reproduction larval rearing and juvenile production of Argyrosomus regius under commercial conditions in Greece. AQUAMEDIT\u20102010 5th International Congress on Aquaculture Fisheries Technology and Environmental Management [abstract] 25th\u201327th November 2010 Technological Education Institute (TEI) Messolonghi Greece."},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006901230367"},{"key":"e_1_2_6_13_1","first-page":"297","volume-title":"Diseases in Asian Aquaculture VI","author":"Labrie L.","year":"2008"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1128\/JCM.37.1.99-102.1999","article-title":"Rapid identification of clinically relevant Nocardia species to genus level by 16S rRNA gene PCR","volume":"37","author":"Laurent F.J.","year":"1999","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1079\/9781845935542.0397"},{"key":"e_1_2_6_16_1","first-page":"129","article-title":"Outbreak of systemic mycosis in intensively reared meagre (Argyrosomus regius)","volume":"2","author":"Manuali E.","year":"2005","journal-title":"Ittiopatologia"},{"key":"e_1_2_6_17_1","unstructured":"MonfortM.C.(2010)Present market situation and prospects of meagre (Argyrosomus regius) as an emerging species in Mediterranean aquaculture. Studies and Reviews no. 89 General Fisheries Commission for the Mediterranean Food and Agriculture Organization of the United Nations (FAO). p. 46."},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2006.00714.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11160-009-9120-7"},{"key":"e_1_2_6_20_1","first-page":"472","volume-title":"Fish Pathology","author":"Roberts R.J","year":"2001"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2010.02.015"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-46-4-314"},{"key":"e_1_2_6_23_1","first-page":"260","article-title":"A study on the enzymatic profiles of the fish pathogen, Nocardia seriolae isolates","volume":"26","author":"Shimahara Y.","year":"2006","journal-title":"Bulletin of the European Association of Fish Pathologists"},{"key":"e_1_2_6_24_1","first-page":"249","article-title":"The study of a tuberculosis\u2010like condition in neon tetras (Hyphessobrycon innesi). II. Characteristics of the bacterium isolated","volume":"16","author":"Valdez I.E.","year":"1963","journal-title":"Microbiologia Espanola"},{"key":"e_1_2_6_25_1","first-page":"483","volume-title":"Merck Veterinary Manual","author":"Walker R.D","year":"1998"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2008.00991.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1128\/jb.173.2.697-703.1991","article-title":"16S ribosomal DNA amplifications for phylogenetic study","volume":"173","author":"Weisberg W.G.","year":"1991","journal-title":"Journal of Bacteriology"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1079\/9781845935542.0000"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjfd.12015","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjfd.12015","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.12015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T17:38:45Z","timestamp":1700674725000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.12015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,24]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1111\/jfd.12015"],"URL":"https:\/\/doi.org\/10.1111\/jfd.12015","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,24]]}}}