{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T22:09:56Z","timestamp":1790287796235,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,11,13]],"date-time":"2013-11-13T00:00:00Z","timestamp":1384300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Spanish Ministerio de Educaci\u00f3n y Cienciaand Ministerio de Ciencia e Innovaci\u00f3n","award":["AGL2006-13158-C03-02"],"award-info":[{"award-number":["AGL2006-13158-C03-02"]}]},{"name":"Spanish Ministerio de Educaci\u00f3n y Cienciaand Ministerio de Ciencia e Innovaci\u00f3n","award":["AGL2009-13282-C02-02"],"award-info":[{"award-number":["AGL2009-13282-C02-02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Enteromyxosis caused by <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>nteromyxum scophthalmi<\/jats:italic> is one of the parasitizations with a higher economic impact on turbot, <jats:italic>Scophthalmus maximus<\/jats:italic> (<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>.), aquaculture. This myxosporean produces severe catarrhal enteritis with abundant inflammatory infiltrates in the lamina propria\u2010submucosa (<jats:styled-content style=\"fixed-case\">LP<\/jats:styled-content>), epithelial detachment and leucocyte depletion of the lymphohaematopoietic organs. Some advances made on the pathogenesis pointed to a role of apoptosis in the enteromyxosis. Therefore, the main aim of this work was to employ <jats:styled-content style=\"fixed-case\">the TUNEL assay<\/jats:styled-content> and the anti\u2010(active caspase\u20103) immunohistochemical assay to detect apoptotic cells in both healthy and <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>.\u00a0scophthalmi<\/jats:italic>\u2010infected turbot in order to establish the presence and distribution of apoptotic cells during development of the disease. More apoptotic cells located within the gastrointestinal epithelium were observed in the initial stages of the infection in <jats:italic>E. scophthalmi<\/jats:italic>\u2010infected turbot compared with non\u2010infected turbot. As the infection progressed, a higher degree of apoptosis occurred in the epithelium of folds heavily parasitized. In the severely infected turbot, apoptosis was also found among the leucocytes of the intestinal inflammatory infiltrates. Moreover, the number of active caspase\u20103\u2010positive cells in the lymphohaematopoietic organs tended to increase with disease severity. In view of the results, increased apoptosis in the epithelium may favour the scaling that occurs during enteromyxosis and cell death of leucocytes in the intestinal <jats:styled-content style=\"fixed-case\">LP<\/jats:styled-content>, contributing to leucocyte depletion in severe cases.<\/jats:p>","DOI":"10.1111\/jfd.12029","type":"journal-article","created":{"date-parts":[[2013,11,13]],"date-time":"2013-11-13T14:07:05Z","timestamp":1384351625000},"page":"21-32","source":"Crossref","is-referenced-by-count":12,"title":["Study of the distribution of active caspase\u20103\u2010positive cells in turbot, <i>Scophthalmus maximus<\/i> (L.), enteromyxosis"],"prefix":"10.1111","volume":"37","author":[{"given":"A P","family":"Losada","sequence":"first","affiliation":[{"name":"Departamento de Ciencias Cl\u00ednicas Veterinarias Facultad de Veterinaria Universidad de Santiago de Compostela  Lugo 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