{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:38:14Z","timestamp":1788327494940,"version":"build-2803163510"},"reference-count":72,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,1,11]],"date-time":"2013-01-11T00:00:00Z","timestamp":1357862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2013,7]]},"abstract":"Abstract<\/jats:title>This study investigates the susceptibilities of the SPB cell line to fish viruses including giant seaperch iridovirus (GSIV<\/jats:styled-content>\u2010K<\/jats:styled-content>1), red sea bream iridovirus (RSIV<\/jats:styled-content>\u2010K<\/jats:styled-content>u), grouper nervous necrosis virus (GNNV<\/jats:styled-content>\u2010K<\/jats:styled-content>1), chum salmon reovirus (CSV<\/jats:styled-content>) and eel herpesvirus (HVA<\/jats:styled-content>). GSIV<\/jats:styled-content>\u2010K<\/jats:styled-content>1, RSIV<\/jats:styled-content>\u2010K<\/jats:styled-content>u and CSV<\/jats:styled-content> replicated well in SPB<\/jats:styled-content> cells, with a significant cytopathic effect and virus production. However, the cells were HVA<\/jats:styled-content> and GNNV<\/jats:styled-content> refractory. To examine the ability of SPB<\/jats:styled-content> cells to stably express foreign protein, expression vectors encoding GNNV B<\/jats:styled-content>1 and B<\/jats:styled-content>2 fused to enhanced green fluorescent protein (EGFP<\/jats:styled-content>) and GSIV ORF<\/jats:styled-content>35L<\/jats:styled-content> fused to D<\/jats:styled-content>sR<\/jats:styled-content>ed were constructed and introduced by transfection into SPB<\/jats:styled-content> cells. Stable transfectants displayed different morphologies compared with SPB<\/jats:styled-content> and with each other. EGFP<\/jats:styled-content>\u2010B<\/jats:styled-content>1 was predominantly localized in the nuclei, EFPF<\/jats:styled-content>\u2010B<\/jats:styled-content>2 was distributed throughout the cytoplasm and nucleus, and granular 35L<\/jats:styled-content>\u2010D<\/jats:styled-content>sR<\/jats:styled-content>ed was localized with secreted vesicles. The expression of EFPF<\/jats:styled-content>\u2010B<\/jats:styled-content>2 in SPB<\/jats:styled-content> cells produced blebs on the surface, but the cells showing stable expression of EGFP<\/jats:styled-content>, EGFP<\/jats:styled-content>\u2010B<\/jats:styled-content>1 or 35L<\/jats:styled-content>\u2010D<\/jats:styled-content>sR<\/jats:styled-content>ed showed normal morphologies. Results show the SPB<\/jats:styled-content> cells and the transfected cells grow well at temperatures between 20 and 35\u00a0\u00b0C and with serum\u2010dependent growth. SPB<\/jats:styled-content> cells are suitable for studies on foreign protein expression and virology.<\/jats:p>","DOI":"10.1111\/jfd.12067","type":"journal-article","created":{"date-parts":[[2013,1,11]],"date-time":"2013-01-11T10:51:02Z","timestamp":1357901462000},"page":"657-667","source":"Crossref","is-referenced-by-count":17,"title":["Viral susceptibility, transfection and growth of SPB<\/scp> \u2013 a fish neural progenitor cell line from the brain of snubnose pompano, T<\/scp>rachinotus blochii<\/i> (L<\/scp>ac\u00e9p\u00e8de)"],"prefix":"10.1111","volume":"36","author":[{"given":"C\u2010M","family":"Wen","sequence":"first","affiliation":[{"name":"Department of Life Sciences National University of Kaohsiung Kaohsiung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u2010C","family":"Ku","sequence":"additional","affiliation":[{"name":"Department of Aquaculture National Penghu University Penghu 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